IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v153y2020icp919-934.html
   My bibliography  Save this article

Novel biocatalyst for optimal biodiesel production from diatoms

Author

Listed:
  • Saranya, G.
  • Ramachandra, T.V.

Abstract

Fatty acid methyl ester (biodiesel) has been derived from oil present in algae through transesterification using catalysts of acids, base, supercritical fluids, etc. These catalysts are corrosive and have been posing challenges of contaminating the environment necessitating environmentally friendly and biodegradable catalysts such as enzyme (lipase) based biocatalysts. In this study, fungal strains (endophytic/free spores) were isolated from an estuarine ecosystem and screened for extracellular lipase activities. A novel fungal strain Cladosporium tenuissimum, identified through molecular technique exhibited higher lipolytic activity among the isolates. The crude lipase extracted from fungus was subjected to ammonium sulphate precipitation and purification using Superdex 200 gel filtration chromatographic system. The molecular weight of purified lipase was found to be ∼46 KDa and a specific activity of 37.2 U/mg. Lipase activities attained stability and reached maximum at 60 °C temperature and pH of 6. The purified enzyme was used as a biological catalyst for enzymatic transesterification of oil obtained from an indigenously isolated salt tolerant diatom Nitzschia punctata. Spectroscopic analysis on fatty acids and Fatty Acid Methyl Esters derived from diatom exhibited similarities in specific functional groups between algal oil and biodiesel. Comparisons on biodiesel yield estimation and FAME compositions of enzyme catalyzed, acid catalyzed biodiesel assessed through gas chromatographic techniques revealed a higher efficiency (87.2 ± 0.47%) of biocatalysts compared to conventional acid catalyst (83.02 ± 0.35%) exhibiting potential scope for large scale application of environment friendly biocatalysts to enhance the conversion performances of the transesterification process.

Suggested Citation

  • Saranya, G. & Ramachandra, T.V., 2020. "Novel biocatalyst for optimal biodiesel production from diatoms," Renewable Energy, Elsevier, vol. 153(C), pages 919-934.
    • Handle: RePEc:eee:renene:v:153:y:2020:i:c:p:919-934
    DOI: 10.1016/j.renene.2020.02.053
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148120302391
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2020.02.053?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Su, Yujie & Song, Kaihui & Zhang, Peidong & Su, Yuqing & Cheng, Jing & Chen, Xiao, 2017. "Progress of microalgae biofuel’s commercialization," Renewable and Sustainable Energy Reviews, Elsevier, vol. 74(C), pages 402-411.
    2. Shahid, Ejaz M. & Jamal, Younis, 2011. "Production of biodiesel: A technical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 15(9), pages 4732-4745.
    3. Veeranan, Theresa & Kasirajaan, Ramachandran & Gurunathan, Baskar & Sahadevan, Renganathan, 2018. "A novel approach for extraction of algal oil from marine macroalgae Ulva fasciata," Renewable Energy, Elsevier, vol. 127(C), pages 64-73.
    4. Guldhe, Abhishek & Singh, Poonam & Kumari, Sheena & Rawat, Ismail & Permaul, Kugen & Bux, Faizal, 2016. "Biodiesel synthesis from microalgae using immobilized Aspergillus niger whole cell lipase biocatalyst," Renewable Energy, Elsevier, vol. 85(C), pages 1002-1010.
    5. Teo, Chee Loong & Jamaluddin, Haryati & Zain, Nur Azimah Mohd & Idris, Ani, 2014. "Biodiesel production via lipase catalysed transesterification of microalgae lipids from Tetraselmis sp," Renewable Energy, Elsevier, vol. 68(C), pages 1-5.
    6. Atabani, A.E. & Silitonga, A.S. & Badruddin, Irfan Anjum & Mahlia, T.M.I. & Masjuki, H.H. & Mekhilef, S., 2012. "A comprehensive review on biodiesel as an alternative energy resource and its characteristics," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(4), pages 2070-2093.
    7. Ramachandra, T.V. & Jain, Rishabh & Krishnadas, Gautham, 2011. "Hotspots of solar potential in India," Renewable and Sustainable Energy Reviews, Elsevier, vol. 15(6), pages 3178-3186, August.
    8. Monique Branco-Vieira & Sergio San Martin & Cristian Agurto & Marco Aurélio dos Santos & Marcos A. V. Freitas & Teresa M. Mata & António A. Martins & Nídia S. Caetano, 2017. "Potential of Phaeodactylum tricornutum for Biodiesel Production under Natural Conditions in Chile," Energies, MDPI, vol. 11(1), pages 1-15, December.
    9. Guo, Yang & Yeh, Thomas & Song, Wenhan & Xu, Donghai & Wang, Shuzhong, 2015. "A review of bio-oil production from hydrothermal liquefaction of algae," Renewable and Sustainable Energy Reviews, Elsevier, vol. 48(C), pages 776-790.
    10. Ramachandra, T.V. & Durga Madhab, Mahapatra & Shilpi, Samantray & Joshi, N.V., 2013. "Algal biofuel from urban wastewater in India: Scope and challenges," Renewable and Sustainable Energy Reviews, Elsevier, vol. 21(C), pages 767-777.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ramachandra, T.V. & Saranya, G., 2022. "Sustainable Bioeconomy prospects of diatom biorefineries in the Indian west coast," Renewable and Sustainable Energy Reviews, Elsevier, vol. 161(C).
    2. Ella Cebisa Linganiso & Boitumelo Tlhaole & Lindokuhle Precious Magagula & Silas Dziike & Linda Zikhona Linganiso & Tshwafo Elias Motaung & Nosipho Moloto & Zikhona Nobuntu Tetana, 2022. "Biodiesel Production from Waste Oils: A South African Outlook," Sustainability, MDPI, vol. 14(4), pages 1-21, February.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Goh, Brandon Han Hoe & Ong, Hwai Chyuan & Cheah, Mei Yee & Chen, Wei-Hsin & Yu, Kai Ling & Mahlia, Teuku Meurah Indra, 2019. "Sustainability of direct biodiesel synthesis from microalgae biomass: A critical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 107(C), pages 59-74.
    2. Lim, Juin Yau & Teng, Sin Yong & How, Bing Shen & Nam, KiJeon & Heo, SungKu & Máša, Vítězslav & Stehlík, Petr & Yoo, Chang Kyoo, 2022. "From microalgae to bioenergy: Identifying optimally integrated biorefinery pathways and harvest scheduling under uncertainties in predicted climate," Renewable and Sustainable Energy Reviews, Elsevier, vol. 168(C).
    3. Azad, A.K. & Rasul, M.G. & Khan, M.M.K. & Sharma, Subhash C. & Mofijur, M. & Bhuiya, M.M.K., 2016. "Prospects, feedstocks and challenges of biodiesel production from beauty leaf oil and castor oil: A nonedible oil sources in Australia," Renewable and Sustainable Energy Reviews, Elsevier, vol. 61(C), pages 302-318.
    4. Bhuiya, M.M.K. & Rasul, M.G. & Khan, M.M.K. & Ashwath, N. & Azad, A.K., 2016. "Prospects of 2nd generation biodiesel as a sustainable fuel—Part: 1 selection of feedstocks, oil extraction techniques and conversion technologies," Renewable and Sustainable Energy Reviews, Elsevier, vol. 55(C), pages 1109-1128.
    5. Mofijur, M. & Masjuki, H.H. & Kalam, M.A. & Ashrafur Rahman, S.M. & Mahmudul, H.M., 2015. "Energy scenario and biofuel policies and targets in ASEAN countries," Renewable and Sustainable Energy Reviews, Elsevier, vol. 46(C), pages 51-61.
    6. Fazril Ideris & Mohd Faiz Muaz Ahmad Zamri & Abd Halim Shamsuddin & Saifuddin Nomanbhay & Fitranto Kusumo & Islam Md Rizwanul Fattah & Teuku Meurah Indra Mahlia, 2022. "Progress on Conventional and Advanced Techniques of In Situ Transesterification of Microalgae Lipids for Biodiesel Production," Energies, MDPI, vol. 15(19), pages 1-32, September.
    7. Patel, Alok & Arora, Neha & Mehtani, Juhi & Pruthi, Vikas & Pruthi, Parul A., 2017. "Assessment of fuel properties on the basis of fatty acid profiles of oleaginous yeast for potential biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 77(C), pages 604-616.
    8. Chamkalani, A. & Zendehboudi, S. & Rezaei, N. & Hawboldt, K., 2020. "A critical review on life cycle analysis of algae biodiesel: current challenges and future prospects," Renewable and Sustainable Energy Reviews, Elsevier, vol. 134(C).
    9. Nitièma-Yefanova, Svitlana & Coniglio, Lucie & Schneider, Raphaël & Nébié, Roger H.C. & Bonzi-Coulibaly, Yvonne L., 2016. "Ethyl biodiesel production from non-edible oils of Balanites aegyptiaca, Azadirachta indica, and Jatropha curcas seeds – Laboratory scale development," Renewable Energy, Elsevier, vol. 96(PA), pages 881-890.
    10. Jia, Guangxin & He, Beibei & Ma, Wenlin & Sun, Yifan, 2019. "Thermodynamic analysis based on simultaneous chemical and phase equilibrium for dehydration of glycerol with methanol," Energy, Elsevier, vol. 188(C).
    11. Sedlar, D. Karasalihović & Vulin, D. & Krajačić, G. & Jukić, L., 2019. "Offshore gas production infrastructure reutilisation for blue energy production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 108(C), pages 159-174.
    12. Noraini, M.Y. & Ong, Hwai Chyuan & Badrul, Mohamed Jan & Chong, W.T., 2014. "A review on potential enzymatic reaction for biofuel production from algae," Renewable and Sustainable Energy Reviews, Elsevier, vol. 39(C), pages 24-34.
    13. Marina Corral Bobadilla & Rubén Lostado Lorza & Rubén Escribano García & Fátima Somovilla Gómez & Eliseo P. Vergara González, 2017. "An Improvement in Biodiesel Production from Waste Cooking Oil by Applying Thought Multi-Response Surface Methodology Using Desirability Functions," Energies, MDPI, vol. 10(1), pages 1-20, January.
    14. Mahmudul, H.M. & Hagos, F.Y. & Mamat, R. & Adam, A. Abdul & Ishak, W.F.W. & Alenezi, R., 2017. "Production, characterization and performance of biodiesel as an alternative fuel in diesel engines – A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 72(C), pages 497-509.
    15. Marta Ramos & Ana Paula Soares Dias & Jaime Filipe Puna & João Gomes & João Carlos Bordado, 2019. "Biodiesel Production Processes and Sustainable Raw Materials," Energies, MDPI, vol. 12(23), pages 1-30, November.
    16. Monteiro, Rodolpho R.C. & Arana-Peña, Sara & da Rocha, Thays N. & Miranda, Letícia P. & Berenguer-Murcia, Ángel & Tardioli, Paulo W. & dos Santos, José C.S. & Fernandez-Lafuente, Roberto, 2021. "Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution?," Renewable Energy, Elsevier, vol. 164(C), pages 1566-1587.
    17. Mat Yasin, Mohd Hafizil & Mamat, Rizalman & Najafi, G. & Ali, Obed Majeed & Yusop, Ahmad Fitri & Ali, Mohd Hafiz, 2017. "Potentials of palm oil as new feedstock oil for a global alternative fuel: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 79(C), pages 1034-1049.
    18. Ramachandra, T.V. & Bajpai, Vishnu & Kulkarni, Gouri & Aithal, Bharath H. & Han, Sun Sheng, 2017. "Economic disparity and CO2 emissions: The domestic energy sector in Greater Bangalore, India," Renewable and Sustainable Energy Reviews, Elsevier, vol. 67(C), pages 1331-1344.
    19. Esonye, Chizoo & Onukwuli, Okechukwu Dominic & Ofoefule, Akuzuo Uwaoma, 2019. "Optimization of methyl ester production from Prunus Amygdalus seed oil using response surface methodology and Artificial Neural Networks," Renewable Energy, Elsevier, vol. 130(C), pages 61-72.
    20. Patel, Alok & Arora, Neha & Sartaj, Km & Pruthi, Vikas & Pruthi, Parul A., 2016. "Sustainable biodiesel production from oleaginous yeasts utilizing hydrolysates of various non-edible lignocellulosic biomasses," Renewable and Sustainable Energy Reviews, Elsevier, vol. 62(C), pages 836-855.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:153:y:2020:i:c:p:919-934. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.