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Efficient lipid extraction from the oleaginous yeast Yarrowia lipolytica using switchable solvents

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  • Yook, Sang Do
  • Kim, Jiwon
  • Woo, Han Min
  • Um, Youngsoon
  • Lee, Sun-Mi

Abstract

Biodiesel production using oleaginous yeasts, such as Yarrowia lipolytica, has recently attracted strong attention in terms of lipid yield and productivity. However, efficient methods of lipid extraction from oleaginous yeasts are required to further improve the economic feasibility of yeast-based biodiesel production. We investigated the effectiveness of selected switchable hydrophilicity solvents (SHSs), namely, N-ethylbutylamine (EB), N-dipropylamine (DP), and N,N-dimethylcyclohexyl-amine (DMCHA), for lipid extraction from the oleaginous yeast Y. lipolytica. Regardless of using harvested cells or culture broth, DMCHA and EB effectively extracted lipids from Y. lipolytica, resulting in up to 13% higher lipid yields than that obtained by conventional organic solvent extraction using a chloroform/methanol mixture. The lipid extracts obtained by the SHSs showed fatty acid compositions comparable to that obtained with the conventional solvent, thus assuring the quality of biodiesel produced through this method. After switching processes by purging with CO2 and N2, the lipids and solvents were successfully recovered, demonstrating the practicability of lipid extraction using SHSs. Consequently, this study provides an effective lipid extraction method for Y. lipolytica that could significantly improve energy efficiency and economic feasibility of an oleaginous-yeast-based biodiesel production process.

Suggested Citation

  • Yook, Sang Do & Kim, Jiwon & Woo, Han Min & Um, Youngsoon & Lee, Sun-Mi, 2019. "Efficient lipid extraction from the oleaginous yeast Yarrowia lipolytica using switchable solvents," Renewable Energy, Elsevier, vol. 132(C), pages 61-67.
    • Handle: RePEc:eee:renene:v:132:y:2019:i:c:p:61-67
    DOI: 10.1016/j.renene.2018.07.129
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    1. Philip G. Jessop & David J. Heldebrant & Xiaowang Li & Charles A. Eckert & Charles L. Liotta, 2005. "Reversible nonpolar-to-polar solvent," Nature, Nature, vol. 436(7054), pages 1102-1102, August.
    2. Dong, Tao & Knoshaug, Eric P. & Pienkos, Philip T. & Laurens, Lieve M.L., 2016. "Lipid recovery from wet oleaginous microbial biomass for biofuel production: A critical review," Applied Energy, Elsevier, vol. 177(C), pages 879-895.
    3. Hou, Jian & Zhang, Peidong & Yuan, Xianzheng & Zheng, Yonghong, 2011. "Life cycle assessment of biodiesel from soybean, jatropha and microalgae in China conditions," Renewable and Sustainable Energy Reviews, Elsevier, vol. 15(9), pages 5081-5091.
    4. Meng, Xin & Yang, Jianming & Xu, Xin & Zhang, Lei & Nie, Qingjuan & Xian, Mo, 2009. "Biodiesel production from oleaginous microorganisms," Renewable Energy, Elsevier, vol. 34(1), pages 1-5.
    5. John Blazeck & Andrew Hill & Leqian Liu & Rebecca Knight & Jarrett Miller & Anny Pan & Peter Otoupal & Hal S. Alper, 2014. "Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production," Nature Communications, Nature, vol. 5(1), pages 1-10, May.
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    1. Bao, Wenjun & Li, Zifu & Wang, Xuemei & Gao, Ruiling & Zhou, Xiaoqin & Cheng, Shikun & Men, Yu & Zheng, Lei, 2021. "Approaches to improve the lipid synthesis of oleaginous yeast Yarrowia lipolytica: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).

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