IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v132y2019icp61-67.html
   My bibliography  Save this article

Efficient lipid extraction from the oleaginous yeast Yarrowia lipolytica using switchable solvents

Author

Listed:
  • Yook, Sang Do
  • Kim, Jiwon
  • Woo, Han Min
  • Um, Youngsoon
  • Lee, Sun-Mi

Abstract

Biodiesel production using oleaginous yeasts, such as Yarrowia lipolytica, has recently attracted strong attention in terms of lipid yield and productivity. However, efficient methods of lipid extraction from oleaginous yeasts are required to further improve the economic feasibility of yeast-based biodiesel production. We investigated the effectiveness of selected switchable hydrophilicity solvents (SHSs), namely, N-ethylbutylamine (EB), N-dipropylamine (DP), and N,N-dimethylcyclohexyl-amine (DMCHA), for lipid extraction from the oleaginous yeast Y. lipolytica. Regardless of using harvested cells or culture broth, DMCHA and EB effectively extracted lipids from Y. lipolytica, resulting in up to 13% higher lipid yields than that obtained by conventional organic solvent extraction using a chloroform/methanol mixture. The lipid extracts obtained by the SHSs showed fatty acid compositions comparable to that obtained with the conventional solvent, thus assuring the quality of biodiesel produced through this method. After switching processes by purging with CO2 and N2, the lipids and solvents were successfully recovered, demonstrating the practicability of lipid extraction using SHSs. Consequently, this study provides an effective lipid extraction method for Y. lipolytica that could significantly improve energy efficiency and economic feasibility of an oleaginous-yeast-based biodiesel production process.

Suggested Citation

  • Yook, Sang Do & Kim, Jiwon & Woo, Han Min & Um, Youngsoon & Lee, Sun-Mi, 2019. "Efficient lipid extraction from the oleaginous yeast Yarrowia lipolytica using switchable solvents," Renewable Energy, Elsevier, vol. 132(C), pages 61-67.
    • Handle: RePEc:eee:renene:v:132:y:2019:i:c:p:61-67
    DOI: 10.1016/j.renene.2018.07.129
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148118309261
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2018.07.129?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Dong, Tao & Knoshaug, Eric P. & Pienkos, Philip T. & Laurens, Lieve M.L., 2016. "Lipid recovery from wet oleaginous microbial biomass for biofuel production: A critical review," Applied Energy, Elsevier, vol. 177(C), pages 879-895.
    2. John Blazeck & Andrew Hill & Leqian Liu & Rebecca Knight & Jarrett Miller & Anny Pan & Peter Otoupal & Hal S. Alper, 2014. "Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production," Nature Communications, Nature, vol. 5(1), pages 1-10, May.
    3. Hou, Jian & Zhang, Peidong & Yuan, Xianzheng & Zheng, Yonghong, 2011. "Life cycle assessment of biodiesel from soybean, jatropha and microalgae in China conditions," Renewable and Sustainable Energy Reviews, Elsevier, vol. 15(9), pages 5081-5091.
    4. Meng, Xin & Yang, Jianming & Xu, Xin & Zhang, Lei & Nie, Qingjuan & Xian, Mo, 2009. "Biodiesel production from oleaginous microorganisms," Renewable Energy, Elsevier, vol. 34(1), pages 1-5.
    5. Philip G. Jessop & David J. Heldebrant & Xiaowang Li & Charles A. Eckert & Charles L. Liotta, 2005. "Reversible nonpolar-to-polar solvent," Nature, Nature, vol. 436(7054), pages 1102-1102, August.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Bao, Wenjun & Li, Zifu & Wang, Xuemei & Gao, Ruiling & Zhou, Xiaoqin & Cheng, Shikun & Men, Yu & Zheng, Lei, 2021. "Approaches to improve the lipid synthesis of oleaginous yeast Yarrowia lipolytica: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Siwina, Siraprapha & Leesing, Ratanaporn, 2021. "Bioconversion of durian (Durio zibethinus Murr.) peel hydrolysate into biodiesel by newly isolated oleaginous yeast Rhodotorula mucilaginosa KKUSY14," Renewable Energy, Elsevier, vol. 163(C), pages 237-245.
    2. Chen, Jiaxin & Li, Ji & Dong, Wenyi & Zhang, Xiaolei & Tyagi, Rajeshwar D. & Drogui, Patrick & Surampalli, Rao Y., 2018. "The potential of microalgae in biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 90(C), pages 336-346.
    3. Robles-Iglesias, Raúl & Naveira-Pazos, Cecilia & Fernández-Blanco, Carla & Veiga, María C. & Kennes, Christian, 2023. "Factors affecting the optimisation and scale-up of lipid accumulation in oleaginous yeasts for sustainable biofuels production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 171(C).
    4. Chamkalani, A. & Zendehboudi, S. & Rezaei, N. & Hawboldt, K., 2020. "A critical review on life cycle analysis of algae biodiesel: current challenges and future prospects," Renewable and Sustainable Energy Reviews, Elsevier, vol. 134(C).
    5. Yellapu, Sravan Kumar & Klai, Nouha & Kaur, Rajwinder & Tyagi, Rajeshwar D. & Surampalli, Rao Y., 2019. "Oleaginous yeast biomass flocculation using bioflocculant produced in wastewater sludge and transesterification using petroleum diesel as a co-solvent," Renewable Energy, Elsevier, vol. 131(C), pages 217-228.
    6. Bao, Wenjun & Li, Zifu & Wang, Xuemei & Gao, Ruiling & Zhou, Xiaoqin & Cheng, Shikun & Men, Yu & Zheng, Lei, 2021. "Approaches to improve the lipid synthesis of oleaginous yeast Yarrowia lipolytica: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).
    7. Zhang, Xiaolei & Yan, Song & Tyagi, Rajeshwar D. & Surampalli, RaoY. & Valéro, Jose R., 2014. "Wastewater sludge as raw material for microbial oils production," Applied Energy, Elsevier, vol. 135(C), pages 192-201.
    8. Sánchez-Bayo, Alejandra & López-Chicharro, Daniel & Morales, Victoria & Espada, Juan José & Puyol, Daniel & Martínez, Fernando & Astals, Sergi & Vicente, Gemma & Bautista, Luis Fernando & Rodríguez, R, 2020. "Biodiesel and biogas production from Isochrysis galbana using dry and wet lipid extraction: A biorefinery approach," Renewable Energy, Elsevier, vol. 146(C), pages 188-195.
    9. Dar, Rouf Ahmad & Tsui, To-Hung & Zhang, Le & Tong, Yen Wah & Sharon, Sigal & Shoseyov, Oded & Liu, Ronghou, 2024. "Fermentation of organic wastes through oleaginous microorganisms for lipid production - Challenges and opportunities," Renewable and Sustainable Energy Reviews, Elsevier, vol. 195(C).
    10. Goh, Brandon Han Hoe & Ong, Hwai Chyuan & Cheah, Mei Yee & Chen, Wei-Hsin & Yu, Kai Ling & Mahlia, Teuku Meurah Indra, 2019. "Sustainability of direct biodiesel synthesis from microalgae biomass: A critical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 107(C), pages 59-74.
    11. Zhang, X.L. & Yan, S. & Tyagi, R.D. & Surampalli, R.Y., 2013. "Biodiesel production from heterotrophic microalgae through transesterification and nanotechnology application in the production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 26(C), pages 216-223.
    12. Dejian Yu & Sun Meng, 2018. "An overview of biomass energy research with bibliometric indicators," Energy & Environment, , vol. 29(4), pages 576-590, June.
    13. Marco Castellini & Stefano Ubertini & Diego Barletta & Ilaria Baffo & Pietro Buzzini & Marco Barbanera, 2021. "Techno-Economic Analysis of Biodiesel Production from Microbial Oil Using Cardoon Stalks as Carbon Source," Energies, MDPI, vol. 14(5), pages 1-21, March.
    14. Ullah, Zahoor & Bustam, Mohamad Azmi & Man, Zakaria, 2015. "Biodiesel production from waste cooking oil by acidic ionic liquid as a catalyst," Renewable Energy, Elsevier, vol. 77(C), pages 521-526.
    15. Nouri, Hoda & Moghimi, Hamid & Nikbakht Rad, Mahzad & Ostovar, Marjan & Farazandeh Mehr, Shima Sadat & Ghanaatian, Fateme & Talebi, Ahmad Farhad, 2019. "Enhanced growth and lipid production in oleaginous fungus, Sarocladium kiliense ADH17: Study on fatty acid profiling and prediction of biodiesel properties," Renewable Energy, Elsevier, vol. 135(C), pages 10-20.
    16. Milazzo, M.F. & Spina, F. & Primerano, P. & Bart, J.C.J., 2013. "Soy biodiesel pathways: Global prospects," Renewable and Sustainable Energy Reviews, Elsevier, vol. 26(C), pages 579-624.
    17. Guldhe, Abhishek & Singh, Poonam & Kumari, Sheena & Rawat, Ismail & Permaul, Kugen & Bux, Faizal, 2016. "Biodiesel synthesis from microalgae using immobilized Aspergillus niger whole cell lipase biocatalyst," Renewable Energy, Elsevier, vol. 85(C), pages 1002-1010.
    18. Mondala, Andro & Hernandez, Rafael & French, Todd & Green, Magan & McFarland, Linda & Ingram, Lonnie, 2015. "Enhanced microbial oil production by activated sludge microorganisms from sugarcane bagasse hydrolyzate," Renewable Energy, Elsevier, vol. 78(C), pages 114-118.
    19. Manzano-Agugliaro, F. & Sanchez-Muros, M.J. & Barroso, F.G. & Martínez-Sánchez, A. & Rojo, S. & Pérez-Bañón, C., 2012. "Insects for biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(6), pages 3744-3753.
    20. Wang, Changbo & Zhang, Lixiao & Chang, Yuan & Pang, Mingyue, 2021. "Energy return on investment (EROI) of biomass conversion systems in China: Meta-analysis focused on system boundary unification," Renewable and Sustainable Energy Reviews, Elsevier, vol. 137(C).

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:132:y:2019:i:c:p:61-67. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.