IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms4131.html
   My bibliography  Save this article

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production

Author

Listed:
  • John Blazeck

    (The University of Texas at Austin)

  • Andrew Hill

    (The University of Texas at Austin)

  • Leqian Liu

    (The University of Texas at Austin)

  • Rebecca Knight

    (Section of Molecular, Cell and Developmental Biology, The University of Texas at Austin)

  • Jarrett Miller

    (The University of Texas at Austin)

  • Anny Pan

    (The University of Texas at Austin)

  • Peter Otoupal

    (The University of Texas at Austin)

  • Hal S. Alper

    (The University of Texas at Austin
    Institute for Cellular and Molecular Biology, The University of Texas at Austin)

Abstract

Economic feasibility of biosynthetic fuel and chemical production hinges upon harnessing metabolism to achieve high titre and yield. Here we report a thorough genotypic and phenotypic optimization of an oleaginous organism to create a strain with significant lipogenesis capability. Specifically, we rewire Yarrowia lipolytica’s native metabolism for superior de novo lipogenesis by coupling combinatorial multiplexing of lipogenesis targets with phenotypic induction. We further complete direct conversion of lipid content into biodiesel. Tri-level metabolic control results in saturated cells containing upwards of 90% lipid content and titres exceeding 25 g l−1 lipids, which represents a 60-fold improvement over parental strain and conditions. Through this rewiring effort, we advance fundamental understanding of lipogenesis, demonstrate non-canonical environmental and intracellular stimuli and uncouple lipogenesis from nitrogen starvation. The high titres and carbon-source independent nature of this lipogenesis in Y. lipolytica highlight the potential of this organism as a platform for efficient oleochemical production.

Suggested Citation

  • John Blazeck & Andrew Hill & Leqian Liu & Rebecca Knight & Jarrett Miller & Anny Pan & Peter Otoupal & Hal S. Alper, 2014. "Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production," Nature Communications, Nature, vol. 5(1), pages 1-10, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4131
    DOI: 10.1038/ncomms4131
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms4131
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms4131?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Dipankar Baisya & Adithya Ramesh & Cory Schwartz & Stefano Lonardi & Ian Wheeldon, 2022. "Genome-wide functional screens enable the prediction of high activity CRISPR-Cas9 and -Cas12a guides in Yarrowia lipolytica," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    2. Bao, Wenjun & Li, Zifu & Wang, Xuemei & Gao, Ruiling & Zhou, Xiaoqin & Cheng, Shikun & Men, Yu & Zheng, Lei, 2021. "Approaches to improve the lipid synthesis of oleaginous yeast Yarrowia lipolytica: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).
    3. Robles-Iglesias, Raúl & Naveira-Pazos, Cecilia & Fernández-Blanco, Carla & Veiga, María C. & Kennes, Christian, 2023. "Factors affecting the optimisation and scale-up of lipid accumulation in oleaginous yeasts for sustainable biofuels production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 171(C).
    4. Shihui Yang & Wei Wang & Hui Wei & Stefanie Van Wychen & Philip T. Pienkos & Min Zhang & Michael E. Himmel, 2016. "Comparison of Nitrogen Depletion and Repletion on Lipid Production in Yeast and Fungal Species," Energies, MDPI, vol. 9(9), pages 1-12, August.
    5. Ko, Ja Kyong & Lee, Jae Hoon & Jung, Je Hyeong & Lee, Sun-Mi, 2020. "Recent advances and future directions in plant and yeast engineering to improve lignocellulosic biofuel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 134(C).
    6. Véra Ehrenstein & Alice Rudge, 2024. "The logic of carbon substitution: from fossilised life to “cell factories”," Review of Agricultural, Food and Environmental Studies, Springer, vol. 105(1), pages 99-123, August.
    7. Svetlana V. Kamzolova & Igor G. Morgunov, 2021. "Physiological, Biochemical and Energetic Characteristics of Torulaspora globosa , a Potential Producer of Biofuel," Energies, MDPI, vol. 14(11), pages 1-9, May.
    8. Yook, Sang Do & Kim, Jiwon & Woo, Han Min & Um, Youngsoon & Lee, Sun-Mi, 2019. "Efficient lipid extraction from the oleaginous yeast Yarrowia lipolytica using switchable solvents," Renewable Energy, Elsevier, vol. 132(C), pages 61-67.
    9. Das, Manali & Patra, Pradipta & Ghosh, Amit, 2020. "Metabolic engineering for enhancing microbial biosynthesis of advanced biofuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 119(C).

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4131. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.