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Enzymatic saccharification and liquid state fermentation of hydrothermal pretreated Tunisian Luffa cylindrica (L.) fibers for cellulosic bioethanol production

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  • Zaafouri, Kaouther
  • Ziadi, Manel
  • ben Hassen-Trabelsi, Aida
  • Mekni, Sabrine
  • Aïssi, Balkiss
  • Alaya, Marwen
  • Hamdi, Moktar

Abstract

The main drivers to develop biorefineries are the energetic and environmental crisis. Consequently, bioenergies have become a scientific and industrial trend. In North Africa, especially in Tunisia, Luffa cylindrica (LC) is a promising energy crop providing lignocellulosic biomass for biofuels production. Three principal fractions compose LC biomass, viz.: α-cellulose (45.8 ± 1.3)%, hemicellulose (20.76 ± 0.3)% and lignins (13.15 ± 0.6)%. The hydrothermal pretreatment of LC fibers was carried out at 96 °C for 54 min. After pretreatment, the reducing sugars amount reached 33.55 g/kg. The subsequent enzymatic saccharification was performed during 1 h at a temperature of 60 °C, by means of two commercial enzymes AP2 and SPC. The enzyme AP2 seemed to be more suitable for the enzymatic hydrolysis of pretreated LC fibers by allowing the release of 59.4 g/kg of reducing sugars, which correspond to a reducing sugars recovery about 93.29%. After that, the liquid state fermentation (LSF) was achieved with Saccharomyces cerevisiae strain during 24 h, in sterile and non-sterile conditions at 30 °C, pH 4.8 ± 0.2 and stirring 250 rpm, in order to conclude about the influence of contamination microflora on fermentation efficiency. After the LSF step, 88.66% of reducing sugars were transformed into alcohol with a conversion rate of 1.58% and a volumetric yield about 70% in sterile conditions. Thus, this work confirms that the potential conversion yield of cellulosic bioethanol is 1 Ton (dry matter) of LC fibers to 13.8545 kg (= 3.6599 Gallon) of biofuel.

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  • Zaafouri, Kaouther & Ziadi, Manel & ben Hassen-Trabelsi, Aida & Mekni, Sabrine & Aïssi, Balkiss & Alaya, Marwen & Hamdi, Moktar, 2017. "Enzymatic saccharification and liquid state fermentation of hydrothermal pretreated Tunisian Luffa cylindrica (L.) fibers for cellulosic bioethanol production," Renewable Energy, Elsevier, vol. 114(PB), pages 1209-1213.
    • Handle: RePEc:eee:renene:v:114:y:2017:i:pb:p:1209-1213
    DOI: 10.1016/j.renene.2017.07.108
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