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Modified simultaneous saccharification and co-fermentation of DLC pretreated corn stover for high-titer cellulosic ethanol production without water washing or detoxifying pretreated biomass

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  • Yuan, Xinchuan
  • Shen, Guannan
  • Chen, Sitong
  • Chen, Xiangxue
  • Zhang, Chengcheng
  • Liu, Shuangmei
  • Jin, Mingjie

Abstract

Corn stover (CS) pretreated by DLC (Densifying Lignocellulosic biomass with acidic Chemicals) pretreatment exhibited higher digestibility and much better fermentability compared to traditional pretreatments. Nevertheless, the xylose fermentation, which was a key for lignocellulosic ethanol production, during SHCF (Separate Hydrolysis and Co-Fermentation) was unsatisfactory at high solid loadings. In this study, modified SSCF (Simultaneous Saccharification and Co-Fermentation) was adopted to promote xylose utilization and ethanol yield. SSCF conditions (temperature, inoculation size, nitrogen source addition) were investigated. A novel strategy “Fed-batch of DLC Biomass and Enzyme (FBE)” was developed for SSCF at 28%–35% solid loadings, which showed higher sugar conversion, higher xylose consumption and superior ethanol yield compared to SHCF and traditional SSCF. The highest ethanol titer of 74.6 g/L produced based on DLC pretreatment was achieved during FBE SSCF without water washing or detoxification of DLC-CS.

Suggested Citation

  • Yuan, Xinchuan & Shen, Guannan & Chen, Sitong & Chen, Xiangxue & Zhang, Chengcheng & Liu, Shuangmei & Jin, Mingjie, 2022. "Modified simultaneous saccharification and co-fermentation of DLC pretreated corn stover for high-titer cellulosic ethanol production without water washing or detoxifying pretreated biomass," Energy, Elsevier, vol. 247(C).
  • Handle: RePEc:eee:energy:v:247:y:2022:i:c:s0360544222003917
    DOI: 10.1016/j.energy.2022.123488
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    References listed on IDEAS

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    1. Kumari, Dolly & Singh, Radhika, 2018. "Pretreatment of lignocellulosic wastes for biofuel production: A critical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 90(C), pages 877-891.
    2. Yuan, Xinchuan & Chen, Xiangxue & Shen, Guannan & Chen, Sitong & Yu, Jianming & Zhai, Rui & Xu, Zhaoxian & Jin, Mingjie, 2022. "Densifying lignocellulosic biomass with sulfuric acid provides a durable feedstock with high digestibility and high fermentability for cellulosic ethanol production," Renewable Energy, Elsevier, vol. 182(C), pages 377-389.
    3. Zabed, H. & Sahu, J.N. & Boyce, A.N. & Faruq, G., 2016. "Fuel ethanol production from lignocellulosic biomass: An overview on feedstocks and technological approaches," Renewable and Sustainable Energy Reviews, Elsevier, vol. 66(C), pages 751-774.
    4. Jonker, J.G.G. & van der Hilst, F. & Junginger, H.M. & Cavalett, O. & Chagas, M.F. & Faaij, A.P.C., 2015. "Outlook for ethanol production costs in Brazil up to 2030, for different biomass crops and industrial technologies," Applied Energy, Elsevier, vol. 147(C), pages 593-610.
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    1. Yu, Jianming & Chen, Sitong & Yu, Yang & Zhang, Chengcheng & Jin, Mingjie, 2024. "Influence of feedstock selection on cellulosic ethanol production based on densified biomass with calcium hydroxide and regular steam pretreatment," Renewable Energy, Elsevier, vol. 227(C).
    2. Xie, Xinyu & Song, Kai & Wang, Jing & Hu, Jinguang & Wu, Shufang & Chu, Qiulu, 2024. "Efficient ethanol production from masson pine sawdust by various organosolv pretreatment and modified pre-hydrolysis simultaneous saccharification and fermentation," Renewable Energy, Elsevier, vol. 225(C).

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