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Isolation and detection of small RNA molecules

Author

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  • J. Fulneček

    (Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic)

Abstract

The article describes in detail methodical procedures for isolation of small RNA molecules, their electrophoresis, northern blotting and finally detection by hybridization with radioactively labeled riboprobes. The presented protocols were successfully introduced and used in our laboratory.

Suggested Citation

  • J. Fulneček, 2007. "Isolation and detection of small RNA molecules," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 53(10), pages 451-455.
    • Handle: RePEc:caa:jnlpse:v:53:y:2007:i:10:id:2196-pse
    DOI: 10.17221/2196-PSE
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    References listed on IDEAS

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    1. Gunter Meister & Thomas Tuschl, 2004. "Mechanisms of gene silencing by double-stranded RNA," Nature, Nature, vol. 431(7006), pages 343-349, September.
    2. Craig C. Mello & Darryl Conte, 2004. "Revealing the world of RNA interference," Nature, Nature, vol. 431(7006), pages 338-342, September.
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