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Dissecting Diagnostic and Management Strategies for Plant Viral Diseases: What Next?

Author

Listed:
  • B. Megala Devi

    (Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India)

  • Samyuktha Guruprasath

    (Department of Chemical and Biotechnology, Sastra Deemed University, Thanjavur 613401, Tamil Nadu, India)

  • Pooraniammal Balu

    (Department of Chemical and Biotechnology, Sastra Deemed University, Thanjavur 613401, Tamil Nadu, India)

  • Anirudha Chattopadhyay

    (Pulses Research Station, S.D. Agricultural University, Sardarkrushinagar 385506, Gujarat, India)

  • Siva Sudha Thilagar

    (Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India)

  • Kanaga Vijayan Dhanabalan

    (Department of Biological Science, Purdue University, West Lafayette, IN 47907, USA)

  • Manoj Choudhary

    (Plant Pathology Department, University of Florida, Gainesville, FL 32611, USA)

  • Swarnalatha Moparthi

    (Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27606, USA)

  • A. Abdul Kader Jailani

    (Plant Pathology Department, University of Florida, Gainesville, FL 32611, USA
    Department of Plant Pathology, North Florida Research and Education Center, University of Florida, Quincy, FL 32351, USA)

Abstract

Recent advancements in molecular biology have revolutionized plant disease diagnosis and management. This review focuses on disease diagnosis through serological techniques, isothermal amplification methods, CRISPR-based approaches, and management strategies using RNA-based methods. Exploring high-throughput sequencing and RNA interference (RNAi) technologies like host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS), this review delves into their potential. Despite the precision offered by RNAi in pest and pathogen management, challenges such as off-target effects and efficient dsRNA delivery persist. This review discusses the significance of these strategies in preventing aphid-mediated plant virus transmission, emphasizing the crucial role of meticulous dsRNA design for effective viral RNA targeting while minimizing harm to plant RNA. Despite acknowledged challenges, including off-target effects and delivery issues, this review underscores the transformative potential of RNA-based strategies in agriculture. Envisaging reduced pesticide dependency and enhanced productivity, these strategies stand as key players in the future of sustainable agriculture.

Suggested Citation

  • B. Megala Devi & Samyuktha Guruprasath & Pooraniammal Balu & Anirudha Chattopadhyay & Siva Sudha Thilagar & Kanaga Vijayan Dhanabalan & Manoj Choudhary & Swarnalatha Moparthi & A. Abdul Kader Jailani, 2024. "Dissecting Diagnostic and Management Strategies for Plant Viral Diseases: What Next?," Agriculture, MDPI, vol. 14(2), pages 1-31, February.
    • Handle: RePEc:gam:jagris:v:14:y:2024:i:2:p:284-:d:1336701
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    References listed on IDEAS

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    1. Craig C. Mello & Darryl Conte, 2004. "Revealing the world of RNA interference," Nature, Nature, vol. 431(7006), pages 338-342, September.
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