IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0193704.html
   My bibliography  Save this article

Citrus aurantium L. dry extracts promote C/ebpβ expression and improve adipocyte differentiation in 3T3-L1 cells

Author

Listed:
  • Gregory Alexander Raciti
  • Francesca Fiory
  • Michele Campitelli
  • Antonella Desiderio
  • Rosa Spinelli
  • Michele Longo
  • Cecilia Nigro
  • Giacomo Pepe
  • Eduardo Sommella
  • Pietro Campiglia
  • Pietro Formisano
  • Francesco Beguinot
  • Claudia Miele

Abstract

Metabolic and/or endocrine dysfunction of the white adipose tissue (WAT) contribute to the development of metabolic disorders, such as Type 2 Diabetes (T2D). Therefore, the identification of products able to improve adipose tissue function represents a valuable strategy for the prevention and/or treatment of T2D. In the current study, we investigated the potential effects of dry extracts obtained from Citrus aurantium L. fruit juice (CAde) on the regulation of 3T3-L1 cells adipocyte differentiation and function in vitro. We found that CAde enhances terminal adipocyte differentiation of 3T3-L1 cells raising the expression of CCAAT/enhancer binding protein beta (C/Ebpβ), peroxisome proliferator activated receptor gamma (Pparγ), glucose transporter type 4 (Glut4) and fatty acid binding protein 4 (Fabp4). CAde improves insulin-induced glucose uptake of 3T3-L1 adipocytes, as well. A focused analysis of the phases occurring in the pre-adipocytes differentiation to mature adipocytes furthermore revealed that CAde promotes the early differentiation stage by up-regulating C/ebpβ expression at 2, 4 and 8 h post the adipogenic induction and anticipating the 3T3-L1 cell cycle entry and progression during mitotic clonal expansion (MCE). These findings provide evidence that the exposure to CAde enhances in vitro fat cell differentiation of pre-adipocytes and functional capacity of mature adipocytes, and pave the way to the development of products derived from Citrus aurantium L. fruit juice, which may improve WAT functional capacity and may be effective for the prevention and/or treatment of T2D.

Suggested Citation

  • Gregory Alexander Raciti & Francesca Fiory & Michele Campitelli & Antonella Desiderio & Rosa Spinelli & Michele Longo & Cecilia Nigro & Giacomo Pepe & Eduardo Sommella & Pietro Campiglia & Pietro Form, 2018. "Citrus aurantium L. dry extracts promote C/ebpβ expression and improve adipocyte differentiation in 3T3-L1 cells," PLOS ONE, Public Library of Science, vol. 13(3), pages 1-20, March.
    • Handle: RePEc:plo:pone00:0193704
    DOI: 10.1371/journal.pone.0193704
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0193704
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0193704&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0193704?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Evan D. Rosen & Bruce M. Spiegelman, 2006. "Adipocytes as regulators of energy balance and glucose homeostasis," Nature, Nature, vol. 444(7121), pages 847-853, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Melkaye G Melka & Michal Abrahamowicz & Gabriel T Leonard & Michel Perron & Louis Richer & Suzanne Veillette & Daniel Gaudet & Tomáš Paus & Zdenka Pausova, 2013. "Clustering of the Metabolic Syndrome Components in Adolescence: Role of Visceral Fat," PLOS ONE, Public Library of Science, vol. 8(12), pages 1-7, December.
    2. Ravikanth Nanduri & Takashi Furusawa & Alexei Lobanov & Bing He & Carol Xie & Kimia Dadkhah & Michael C. Kelly & Oksana Gavrilova & Frank J. Gonzalez & Michael Bustin, 2022. "Epigenetic regulation of white adipose tissue plasticity and energy metabolism by nucleosome binding HMGN proteins," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Meiqi Fan & Eun-Kyung Kim & Young-Jin Choi & Yujiao Tang & Sang-Ho Moon, 2019. "The Role of Momordica charantia in Resisting Obesity," IJERPH, MDPI, vol. 16(18), pages 1-17, September.
    4. Cheng Xiao & Elke Albrecht & Dirk Dannenberger & Weibo Kong & Hao Gu & Harald M. Hammon & Steffen Maak, 2024. "Effects of Supplementation with Essential Fatty Acids and Conjugated Linoleic Acids on Muscle Structure and Fat Deposition in Lactating Holstein Cows," Agriculture, MDPI, vol. 14(10), pages 1-18, September.
    5. YunSook Jung & Ji-Hye Kim & Ah-Ra Shin & Keun-Bae Song & Atsuo Amano & Youn-Hee Choi, 2023. "Association of Adiposity with Periodontitis and Metabolic Syndrome: From the Third National Health and Nutrition Examination Survey of United States," IJERPH, MDPI, vol. 20(3), pages 1-9, January.
    6. Stephen J. Gaudino & Ankita Singh & Huakang Huang & Jyothi Padiadpu & Makheni Jean-Pierre & Cody Kempen & Tej Bahadur & Kiyoshi Shiomitsu & Richard Blumberg & Kenneth R. Shroyer & Semir Beyaz & Natali, 2024. "Intestinal IL-22RA1 signaling regulates intrinsic and systemic lipid and glucose metabolism to alleviate obesity-associated disorders," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    7. Ewa Bielczyk-Maczynska & Meng Zhao & Peter-James H. Zushin & Theresia M. Schnurr & Hyun-Jung Kim & Jiehan Li & Pratima Nallagatla & Panjamaporn Sangwung & Chong Y. Park & Cameron Cornn & Andreas Stahl, 2022. "G protein-coupled receptor 151 regulates glucose metabolism and hepatic gluconeogenesis," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    8. Motohiro Sekiya & Kenta Kainoh & Takehito Sugasawa & Ryunosuke Yoshino & Takatsugu Hirokawa & Hiroaki Tokiwa & Shogo Nakano & Satoru Nagatoishi & Kouhei Tsumoto & Yoshinori Takeuchi & Takafumi Miyamot, 2021. "The transcriptional corepressor CtBP2 serves as a metabolite sensor orchestrating hepatic glucose and lipid homeostasis," Nature Communications, Nature, vol. 12(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0193704. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.