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CENP-C/H/I/K/M/T/W/N/L and hMis12 but not CENP-S/X participate in complex formation in the nucleoplasm of living human interphase cells outside centromeres

Author

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  • Christian Hoischen
  • Sibel Yavas
  • Thorsten Wohland
  • Stephan Diekmann

Abstract

Kinetochore proteins assemble onto centromeric chromatin and regulate DNA segregation during cell division. The inner kinetochore proteins bind centromeres while most outer kinetochore proteins assemble at centromeres during mitosis, connecting the complex to microtubules. Here, we measured the co-migration between protein pairs of the constitutive centromere associated network (CCAN) and hMis12 complexes by fluorescence cross-correlation spectroscopy (FCCS) in the nucleoplasm outside centromeres in living human interphase cells. FCCS is a method that can tell if in living cells two differently fluorescently labelled molecules migrate independently, or co-migrate and thus are part of one and the same soluble complex. We also determined the apparent dissociation constants (Kd) of the hetero-dimers CENP-T/W and CENP-S/X. We measured co-migration between CENP-K and CENP-T as well as between CENP-M and CENP-T but not between CENP-T/W and CENP-S/X. Furthermore, CENP-C co-migrated with CENP-H, and CENP-K with CENP-N as well as with CENP-L. Thus, in the nucleoplasm outside centromeres, a large fraction of the CENP-H/I/K/M proteins interact with CENP-C, CENP-N/L and CENP-T/W but not with CENP-S/X. Our FCCS analysis of the Mis12 complex showed that hMis12, Nsl1, Dsn1 and Nnf1 also form a complex outside centromeres of which at least hMis12 associated with the CENP-C/H/I/K/M/T/W/N/L complex.

Suggested Citation

  • Christian Hoischen & Sibel Yavas & Thorsten Wohland & Stephan Diekmann, 2018. "CENP-C/H/I/K/M/T/W/N/L and hMis12 but not CENP-S/X participate in complex formation in the nucleoplasm of living human interphase cells outside centromeres," PLOS ONE, Public Library of Science, vol. 13(3), pages 1-26, March.
  • Handle: RePEc:plo:pone00:0192572
    DOI: 10.1371/journal.pone.0192572
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    References listed on IDEAS

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    3. Ben E. Black & Daniel R. Foltz & Srinivas Chakravarthy & Karolin Luger & Virgil L. Woods & Don W. Cleveland, 2004. "Structural determinants for generating centromeric chromatin," Nature, Nature, vol. 430(6999), pages 578-582, July.
    4. Lucie Y. Guo & Praveen Kumar Allu & Levani Zandarashvili & Kara L. McKinley & Nikolina Sekulic & Jennine M. Dawicki-McKenna & Daniele Fachinetti & Glennis A. Logsdon & Ryan M. Jamiolkowski & Don W. Cl, 2017. "Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition," Nature Communications, Nature, vol. 8(1), pages 1-15, August.
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