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In vitro centromere and kinetochore assembly on defined chromatin templates

Author

Listed:
  • Annika Guse

    (Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA)

  • Christopher W. Carroll

    (Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA)

  • Ben Moree

    (Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA)

  • Colin J. Fuller

    (Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA)

  • Aaron F. Straight

    (Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA)

Abstract

In vitro reconstitution of the centromere Centromeres are specialized chromatin domains that are essential for chromosome segregation and cell division, but it is unclear how they are established and assembled to generate microtubule-binding sites, or kinetochores, during mitosis. Here, Aaron Straight and colleagues have reconstituted vertebrate centromeric chromatin in vitro and show that, when added to cell-free extracts, this reconstituted CENP-A chromatin is sufficient for the assembly of centromere and kinetochore proteins, binding of microtubules and mitotic checkpoint function. This cell-free system is used to dissect various molecular features of CENP-A, and should be a valuable approach to study the complex centromeres of vertebrates.

Suggested Citation

  • Annika Guse & Christopher W. Carroll & Ben Moree & Colin J. Fuller & Aaron F. Straight, 2011. "In vitro centromere and kinetochore assembly on defined chromatin templates," Nature, Nature, vol. 477(7364), pages 354-358, September.
    • Handle: RePEc:nat:nature:v:477:y:2011:i:7364:d:10.1038_nature10379
    DOI: 10.1038/nature10379
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    Cited by:

    1. Christian Hoischen & Sibel Yavas & Thorsten Wohland & Stephan Diekmann, 2018. "CENP-C/H/I/K/M/T/W/N/L and hMis12 but not CENP-S/X participate in complex formation in the nucleoplasm of living human interphase cells outside centromeres," PLOS ONE, Public Library of Science, vol. 13(3), pages 1-26, March.

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