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Crystal structure of the human centromeric nucleosome containing CENP-A

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  • Hiroaki Tachiwana

    (Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan)

  • Wataru Kagawa

    (Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan)

  • Tatsuya Shiga

    (Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan)

  • Akihisa Osakabe

    (Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan)

  • Yuta Miya

    (Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan)

  • Kengo Saito

    (Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan)

  • Yoko Hayashi-Takanaka

    (Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan)

  • Takashi Oda

    (Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan)

  • Mamoru Sato

    (Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan)

  • Sam-Yong Park

    (Protein Design Laboratory, Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan)

  • Hiroshi Kimura

    (Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan)

  • Hitoshi Kurumizaka

    (Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan)

Abstract

The centromeric nucleosome Centromeres are epigenetically marked by the assembly of nucleosomes containing CENP-A, a centromere-specific histone H3 variant. Tachiwana et al. report the crystal structure of the human centromeric nucleosome bound to DNA. They find a canonical arrangement of an octamer of histone proteins with DNA wrapped in a left-handed superhelix. There is flexibility in the DNA regions at the entrance and exit of the nucleosome, and a loop in CENP-A may help to stabilize its incorporation into centromeric chromatin. As the first view of the CENP-A-containing nucleosome, the structure helps to clarify various models that have been debated in the literature.

Suggested Citation

  • Hiroaki Tachiwana & Wataru Kagawa & Tatsuya Shiga & Akihisa Osakabe & Yuta Miya & Kengo Saito & Yoko Hayashi-Takanaka & Takashi Oda & Mamoru Sato & Sam-Yong Park & Hiroshi Kimura & Hitoshi Kurumizaka, 2011. "Crystal structure of the human centromeric nucleosome containing CENP-A," Nature, Nature, vol. 476(7359), pages 232-235, August.
    • Handle: RePEc:nat:nature:v:476:y:2011:i:7359:d:10.1038_nature10258
    DOI: 10.1038/nature10258
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    Cited by:

    1. Harsh Nagpal & Ahmad Ali-Ahmad & Yasuhiro Hirano & Wei Cai & Mario Halic & Tatsuo Fukagawa & Nikolina Sekulić & Beat Fierz, 2023. "CENP-A and CENP-B collaborate to create an open centromeric chromatin state," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Christian Hoischen & Sibel Yavas & Thorsten Wohland & Stephan Diekmann, 2018. "CENP-C/H/I/K/M/T/W/N/L and hMis12 but not CENP-S/X participate in complex formation in the nucleoplasm of living human interphase cells outside centromeres," PLOS ONE, Public Library of Science, vol. 13(3), pages 1-26, March.

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