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Gut Dysbiosis in Patients with Anorexia Nervosa

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  • Chihiro Morita
  • Hirokazu Tsuji
  • Tomokazu Hata
  • Motoharu Gondo
  • Shu Takakura
  • Keisuke Kawai
  • Kazufumi Yoshihara
  • Kiyohito Ogata
  • Koji Nomoto
  • Kouji Miyazaki
  • Nobuyuki Sudo

Abstract

Anorexia nervosa (AN) is a psychological illness with devastating physical consequences; however, its pathophysiological mechanism remains unclear. Because numerous reports have indicated the importance of gut microbiota in the regulation of weight gain, it is reasonable to speculate that AN patients might have a microbial imbalance, i.e. dysbiosis, in their gut. In this study, we compared the fecal microbiota of female patients with AN (n = 25), including restrictive (ANR, n = 14) and binge-eating (ANBP, n = 11) subtypes, with those of age-matched healthy female controls (n = 21) using the Yakult Intestinal Flora-SCAN based on 16S or 23S rRNA–targeted RT–quantitative PCR technology. AN patients had significantly lower amounts of total bacteria and obligate anaerobes including those from the Clostridium coccoides group, Clostridium leptum subgroup, and Bacteroides fragilis group than the age-matched healthy women. Lower numbers of Streptococcus were also found in the AN group than in the control group. In the analysis based on AN subtypes, the counts of the Bacteroides fragilis group in the ANR and ANBP groups and the counts of the Clostridium coccoides group in the ANR group were significantly lower than those in the control group. The detection rate of the Lactobacillus plantarum subgroup was significantly lower in the AN group than in the control group. The AN group had significantly lower acetic and propionic acid concentrations in the feces than the control group. Moreover, the subtype analysis showed that the fecal concentrations of acetic acid were lower in the ANR group than in the control group. Principal component analysis confirmed a clear difference in the bacterial components between the AN patients and healthy women. Collectively, these results clearly indicate the existence of dysbiosis in the gut of AN patients.

Suggested Citation

  • Chihiro Morita & Hirokazu Tsuji & Tomokazu Hata & Motoharu Gondo & Shu Takakura & Keisuke Kawai & Kazufumi Yoshihara & Kiyohito Ogata & Koji Nomoto & Kouji Miyazaki & Nobuyuki Sudo, 2015. "Gut Dysbiosis in Patients with Anorexia Nervosa," PLOS ONE, Public Library of Science, vol. 10(12), pages 1-13, December.
    • Handle: RePEc:plo:pone00:0145274
    DOI: 10.1371/journal.pone.0145274
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    References listed on IDEAS

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    2. Peter J. Turnbaugh & Micah Hamady & Tanya Yatsunenko & Brandi L. Cantarel & Alexis Duncan & Ruth E. Ley & Mitchell L. Sogin & William J. Jones & Bruce A. Roe & Jason P. Affourtit & Michael Egholm & Be, 2009. "A core gut microbiome in obese and lean twins," Nature, Nature, vol. 457(7228), pages 480-484, January.
    3. Ruth E. Ley & Peter J. Turnbaugh & Samuel Klein & Jeffrey I. Gordon, 2006. "Human gut microbes associated with obesity," Nature, Nature, vol. 444(7122), pages 1022-1023, December.
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