IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0145274.html
   My bibliography  Save this article

Gut Dysbiosis in Patients with Anorexia Nervosa

Author

Listed:
  • Chihiro Morita
  • Hirokazu Tsuji
  • Tomokazu Hata
  • Motoharu Gondo
  • Shu Takakura
  • Keisuke Kawai
  • Kazufumi Yoshihara
  • Kiyohito Ogata
  • Koji Nomoto
  • Kouji Miyazaki
  • Nobuyuki Sudo

Abstract

Anorexia nervosa (AN) is a psychological illness with devastating physical consequences; however, its pathophysiological mechanism remains unclear. Because numerous reports have indicated the importance of gut microbiota in the regulation of weight gain, it is reasonable to speculate that AN patients might have a microbial imbalance, i.e. dysbiosis, in their gut. In this study, we compared the fecal microbiota of female patients with AN (n = 25), including restrictive (ANR, n = 14) and binge-eating (ANBP, n = 11) subtypes, with those of age-matched healthy female controls (n = 21) using the Yakult Intestinal Flora-SCAN based on 16S or 23S rRNA–targeted RT–quantitative PCR technology. AN patients had significantly lower amounts of total bacteria and obligate anaerobes including those from the Clostridium coccoides group, Clostridium leptum subgroup, and Bacteroides fragilis group than the age-matched healthy women. Lower numbers of Streptococcus were also found in the AN group than in the control group. In the analysis based on AN subtypes, the counts of the Bacteroides fragilis group in the ANR and ANBP groups and the counts of the Clostridium coccoides group in the ANR group were significantly lower than those in the control group. The detection rate of the Lactobacillus plantarum subgroup was significantly lower in the AN group than in the control group. The AN group had significantly lower acetic and propionic acid concentrations in the feces than the control group. Moreover, the subtype analysis showed that the fecal concentrations of acetic acid were lower in the ANR group than in the control group. Principal component analysis confirmed a clear difference in the bacterial components between the AN patients and healthy women. Collectively, these results clearly indicate the existence of dysbiosis in the gut of AN patients.

Suggested Citation

  • Chihiro Morita & Hirokazu Tsuji & Tomokazu Hata & Motoharu Gondo & Shu Takakura & Keisuke Kawai & Kazufumi Yoshihara & Kiyohito Ogata & Koji Nomoto & Kouji Miyazaki & Nobuyuki Sudo, 2015. "Gut Dysbiosis in Patients with Anorexia Nervosa," PLOS ONE, Public Library of Science, vol. 10(12), pages 1-13, December.
    • Handle: RePEc:plo:pone00:0145274
    DOI: 10.1371/journal.pone.0145274
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145274
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145274&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0145274?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Peter J. Turnbaugh & Micah Hamady & Tanya Yatsunenko & Brandi L. Cantarel & Alexis Duncan & Ruth E. Ley & Mitchell L. Sogin & William J. Jones & Bruce A. Roe & Jason P. Affourtit & Michael Egholm & Be, 2009. "A core gut microbiome in obese and lean twins," Nature, Nature, vol. 457(7228), pages 480-484, January.
    2. Ruth E. Ley & Peter J. Turnbaugh & Samuel Klein & Jeffrey I. Gordon, 2006. "Human gut microbes associated with obesity," Nature, Nature, vol. 444(7122), pages 1022-1023, December.
    3. Peter J. Turnbaugh & Ruth E. Ley & Michael A. Mahowald & Vincent Magrini & Elaine R. Mardis & Jeffrey I. Gordon, 2006. "An obesity-associated gut microbiome with increased capacity for energy harvest," Nature, Nature, vol. 444(7122), pages 1027-1031, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Hannah Lees & Jonathan Swann & Simon M Poucher & Jeremy K Nicholson & Elaine Holmes & Ian D Wilson & Julian R Marchesi, 2014. "Age and Microenvironment Outweigh Genetic Influence on the Zucker Rat Microbiome," PLOS ONE, Public Library of Science, vol. 9(9), pages 1-11, September.
    2. Dongyang Yang & Wei Xu, 2023. "Estimation of Mediation Effect on Zero-Inflated Microbiome Mediators," Mathematics, MDPI, vol. 11(13), pages 1-16, June.
    3. Jeffrey D Galley & Michael Bailey & Claire Kamp Dush & Sarah Schoppe-Sullivan & Lisa M Christian, 2014. "Maternal Obesity Is Associated with Alterations in the Gut Microbiome in Toddlers," PLOS ONE, Public Library of Science, vol. 9(11), pages 1-13, November.
    4. Kiran Konain & Sadia & Turfa Nadeem & Adeed Khan & Warda Iqbal & Arsalan & Amir Javed & Ruby Khan & Kainat Jamil & Kainat Jamil, 2018. "Importance of Probiotics in Gastrointestinal Tract," Journal of Asian Scientific Research, Asian Economic and Social Society, vol. 8(3), pages 128-143, March.
    5. Pirjo Wacklin & Harri Mäkivuokko & Noora Alakulppi & Janne Nikkilä & Heli Tenkanen & Jarkko Räbinä & Jukka Partanen & Kari Aranko & Jaana Mättö, 2011. "Secretor Genotype (FUT2 gene) Is Strongly Associated with the Composition of Bifidobacteria in the Human Intestine," PLOS ONE, Public Library of Science, vol. 6(5), pages 1-10, May.
    6. Mariana F. Fernández & Iris Reina-Pérez & Juan Manuel Astorga & Andrea Rodríguez-Carrillo & Julio Plaza-Díaz & Luis Fontana, 2018. "Breast Cancer and Its Relationship with the Microbiota," IJERPH, MDPI, vol. 15(8), pages 1-20, August.
    7. Vinod Nikhra, 2019. "The Novel Dimensions of Cardio-Metabolic Health Gut Microbiota, Dysbiosis and its Fallouts," Current Research in Diabetes & Obesity Journal, Juniper Publishers Inc., vol. 11(1), pages 28-37, June.
    8. Shinji Fukuda & Yumiko Nakanishi & Eisuke Chikayama & Hiroshi Ohno & Tsuneo Hino & Jun Kikuchi, 2009. "Evaluation and Characterization of Bacterial Metabolic Dynamics with a Novel Profiling Technique, Real-Time Metabolotyping," PLOS ONE, Public Library of Science, vol. 4(3), pages 1-10, March.
    9. Zemin Zheng & Jinchi Lv & Wei Lin, 2021. "Nonsparse Learning with Latent Variables," Operations Research, INFORMS, vol. 69(1), pages 346-359, January.
    10. Michael DiMarzio & Brigida Rusconi & Neela H Yennawar & Mark Eppinger & Andrew D Patterson & Edward G Dudley, 2017. "Identification of a mouse Lactobacillus johnsonii strain with deconjugase activity against the FXR antagonist T-β-MCA," PLOS ONE, Public Library of Science, vol. 12(9), pages 1-15, September.
    11. Tamar Ringel-Kulka & Jing Cheng & Yehuda Ringel & Jarkko Salojärvi & Ian Carroll & Airi Palva & Willem M de Vos & Reetta Satokari, 2013. "Intestinal Microbiota in Healthy U.S. Young Children and Adults—A High Throughput Microarray Analysis," PLOS ONE, Public Library of Science, vol. 8(5), pages 1-10, May.
    12. Liat Shenhav & Ori Furman & Leah Briscoe & Mike Thompson & Justin D Silverman & Itzhak Mizrahi & Eran Halperin, 2019. "Modeling the temporal dynamics of the gut microbial community in adults and infants," PLOS Computational Biology, Public Library of Science, vol. 15(6), pages 1-21, June.
    13. Daniel Beck & James A Foster, 2014. "Machine Learning Techniques Accurately Classify Microbial Communities by Bacterial Vaginosis Characteristics," PLOS ONE, Public Library of Science, vol. 9(2), pages 1-8, February.
    14. Feng Tong & Teng Wang & Na L. Gao & Ziying Liu & Kuiqing Cui & Yiqian Duan & Sicheng Wu & Yuhong Luo & Zhipeng Li & Chengjian Yang & Yixue Xu & Bo Lin & Liguo Yang & Alfredo Pauciullo & Deshun Shi & G, 2022. "The microbiome of the buffalo digestive tract," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    15. Koji Hosomi & Mayu Saito & Jonguk Park & Haruka Murakami & Naoko Shibata & Masahiro Ando & Takahiro Nagatake & Kana Konishi & Harumi Ohno & Kumpei Tanisawa & Attayeb Mohsen & Yi-An Chen & Hitoshi Kawa, 2022. "Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    16. Gertrude Ecklu-Mensah & Candice Choo-Kang & Maria Gjerstad Maseng & Sonya Donato & Pascal Bovet & Bharathi Viswanathan & Kweku Bedu-Addo & Jacob Plange-Rhule & Prince Oti Boateng & Terrence E. Forrest, 2023. "Gut microbiota and fecal short chain fatty acids differ with adiposity and country of origin: the METS-microbiome study," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    17. Małgorzata Moszak & Monika Szulińska & Marta Walczak-Gałęzewska & Paweł Bogdański, 2021. "Nutritional Approach Targeting Gut Microbiota in NAFLD—To Date," IJERPH, MDPI, vol. 18(4), pages 1-32, February.
    18. Marina Sanchez & Shirin Panahi & Angelo Tremblay, 2014. "Childhood Obesity: A Role for Gut Microbiota?," IJERPH, MDPI, vol. 12(1), pages 1-14, December.
    19. Xiuying Zhang & Dongqian Shen & Zhiwei Fang & Zhuye Jie & Xinmin Qiu & Chunfang Zhang & Yingli Chen & Linong Ji, 2013. "Human Gut Microbiota Changes Reveal the Progression of Glucose Intolerance," PLOS ONE, Public Library of Science, vol. 8(8), pages 1-11, August.
    20. Alessandra N. Bazzano & Kaitlin S. Potts & Lydia A. Bazzano & John B. Mason, 2017. "The Life Course Implications of Ready to Use Therapeutic Food for Children in Low-Income Countries," IJERPH, MDPI, vol. 14(4), pages 1-19, April.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0145274. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.