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18F-FDG PET Imaging of Murine Atherosclerosis: Association with Gene Expression of Key Molecular Markers

Author

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  • Anne Mette Fisker Hag
  • Sune Folke Pedersen
  • Christina Christoffersen
  • Tina Binderup
  • Mette Munk Jensen
  • Jesper Tranekjær Jørgensen
  • Dorthe Skovgaard
  • Rasmus Sejersten Ripa
  • Andreas Kjaer

Abstract

Aim: To study whether 18F-FDG can be used for in vivo imaging of atherogenesis by examining the correlation between 18F-FDG uptake and gene expression of key molecular markers of atherosclerosis in apoE−/− mice. Methods: Nine groups of apoE−/− mice were given normal chow or high-fat diet. At different time-points, 18F-FDG PET/contrast-enhanced CT scans were performed on dedicated animal scanners. After scans, animals were euthanized, aortas removed, gamma counted, RNA extracted from the tissue, and gene expression of chemo (C-X-C motif) ligand 1 (CXCL-1), monocyte chemoattractant protein (MCP)-1, vascular cell adhesion molecule (VCAM)-1, cluster of differentiation molecule (CD)-68, osteopontin (OPN), lectin-like oxidized LDL-receptor (LOX)-1, hypoxia-inducible factor (HIF)-1α, HIF-2α, vascular endothelial growth factor A (VEGF), and tissue factor (TF) was measured by means of qPCR. Results: The uptake of 18F-FDG increased over time in the groups of mice receiving high-fat diet measured by PET and ex vivo gamma counting. The gene expression of all examined markers of atherosclerosis correlated significantly with 18F-FDG uptake. The strongest correlation was seen with TF and CD68 (p

Suggested Citation

  • Anne Mette Fisker Hag & Sune Folke Pedersen & Christina Christoffersen & Tina Binderup & Mette Munk Jensen & Jesper Tranekjær Jørgensen & Dorthe Skovgaard & Rasmus Sejersten Ripa & Andreas Kjaer, 2012. "18F-FDG PET Imaging of Murine Atherosclerosis: Association with Gene Expression of Key Molecular Markers," PLOS ONE, Public Library of Science, vol. 7(11), pages 1-9, November.
    • Handle: RePEc:plo:pone00:0050908
    DOI: 10.1371/journal.pone.0050908
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    1. Peter Libby, 2002. "Inflammation in atherosclerosis," Nature, Nature, vol. 420(6917), pages 868-874, December.
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