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Translaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex

Author

Listed:
  • John Vargas-Ortiz

    (Yale School of Medicine)

  • Lin Lin

    (Yale School of Medicine)

  • Vena K. Martinez

    (Yale School of Medicine)

  • Rong-jian Liu

    (Yale School of Medicine)

  • Rachel Babij

    (Weill Cornell Medicine
    Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program)

  • Zhe Ran S. Duan

    (Weill Cornell Medicine
    Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program)

  • Sam Wacks

    (Weill Cornell Medicine)

  • Liyuan Sun

    (Yale School of Medicine)

  • Amanda Wang

    (Yale School of Medicine)

  • Sajida Khan

    (Yale School of Medicine)

  • John Lee Soto-Vargas

    (Yale School of Medicine)

  • Natalia V. Marco García

    (Weill Cornell Medicine)

  • Alicia Che

    (Yale School of Medicine
    Wu Tsai Institute, Yale University)

Abstract

Synchronous neuronal activity is a hallmark of the developing mouse primary somatosensory cortex. While the patterns of synchronous neuronal activity in cortical layer 2/3 have been well described, the source of the robust layer 2/3 activity is still unknown. Using a novel microprism preparation and in vivo 2-photon imaging in neonatal mice, we show that synchronous neuronal activity is organized in barrel columns across layers. Monosynaptic rabies tracing and slice electrophysiology experiments reveal that layer 2/3 pyramidal neurons receive significant layer 5 inputs during the first postnatal week, and silencing layer 5 synaptic outputs results in a significant reduction in spontaneous activity, abnormal sensory-evoked activity and disrupted layer 4-layer 2/3 connectivity. Our results demonstrate that translaminar layer 5-layer 2/3 connectivity plays an important role in synchronizing the developing barrel column to ensure the strengthening of layer 4-layer 2/3 connections, supporting the formation of the canonical cortical organization in barrel cortex.

Suggested Citation

  • John Vargas-Ortiz & Lin Lin & Vena K. Martinez & Rong-jian Liu & Rachel Babij & Zhe Ran S. Duan & Sam Wacks & Liyuan Sun & Amanda Wang & Sajida Khan & John Lee Soto-Vargas & Natalia V. Marco García & , 2025. "Translaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex," Nature Communications, Nature, vol. 16(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-024-55783-w
    DOI: 10.1038/s41467-024-55783-w
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