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Magnetically reshapable 3D multi-electrode arrays of liquid metals for electrophysiological analysis of brain organoids

Author

Listed:
  • Enji Kim

    (Yonsei University
    Yonsei University)

  • Eunseon Jeong

    (Yonsei University)

  • Yeon-Mi Hong

    (Yonsei University
    Yonsei University)

  • Inhea Jeong

    (Yonsei University
    Yonsei University)

  • Junghoon Kim

    (Yonsei University
    Yonsei University)

  • Yong Won Kwon

    (Yonsei University
    Yonsei University)

  • Young-Geun Park

    (Yonsei University
    Yonsei University)

  • Jiin Lee

    (Yonsei University)

  • Suah Choi

    (Yonsei University)

  • Ju-Young Kim

    (Yonsei University
    Yonsei University)

  • Jae-Hyun Lee

    (Yonsei University
    Yonsei University)

  • Seung-Woo Cho

    (Yonsei University
    Yonsei University
    Yonsei University)

  • Jang-Ung Park

    (Yonsei University
    Yonsei University
    Yonsei University
    Yonsei University College of Medicine)

Abstract

To comprehend the volumetric neural connectivity of a brain organoid, it is crucial to monitor the spatiotemporal electrophysiological signals within the organoid, known as intra-organoid signals. However, previous methods risked damaging the three-dimensional (3D) cytoarchitecture of organoids, either through sectioning or inserting rigid needle-like electrodes. Also, the limited numbers of electrodes in fixed positions with non-adjustable electrode shapes were insufficient for examining the complex neural activity throughout the organoid. Herein, we present a magnetically reshapable 3D multi-electrode array (MEA) using direct printing of liquid metals for electrophysiological analysis of brain organoids. The adaptable distribution and the softness of these printed electrodes facilitate the spatiotemporal recording of intra-organoid signals. Furthermore, the unique capability to reshape these soft electrodes within the organoid using magnetic fields allows a single electrode in the MEA to record from multiple points, effectively increasing the recording site density without the need for additional electrodes.

Suggested Citation

  • Enji Kim & Eunseon Jeong & Yeon-Mi Hong & Inhea Jeong & Junghoon Kim & Yong Won Kwon & Young-Geun Park & Jiin Lee & Suah Choi & Ju-Young Kim & Jae-Hyun Lee & Seung-Woo Cho & Jang-Ung Park, 2025. "Magnetically reshapable 3D multi-electrode arrays of liquid metals for electrophysiological analysis of brain organoids," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-024-55752-3
    DOI: 10.1038/s41467-024-55752-3
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    References listed on IDEAS

    as
    1. Hyogeun Shin & Sohyeon Jeong & Ju-Hyun Lee & Woong Sun & Nakwon Choi & Il-Joo Cho, 2021. "3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    2. Paul R. Ormel & Renata Vieira de Sá & Emma J. Bodegraven & Henk Karst & Oliver Harschnitz & Marjolein A. M. Sneeboer & Lill Eva Johansen & Roland E. Dijk & Nicky Scheefhals & Amber Berdenis van Berlek, 2018. "Microglia innately develop within cerebral organoids," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    3. Giorgia Quadrato & Tuan Nguyen & Evan Z. Macosko & John L. Sherwood & Sung Min Yang & Daniel R. Berger & Natalie Maria & Jorg Scholvin & Melissa Goldman & Justin P. Kinney & Edward S. Boyden & Jeff W., 2017. "Cell diversity and network dynamics in photosensitive human brain organoids," Nature, Nature, vol. 545(7652), pages 48-53, May.
    4. Tal Sharf & Tjitse Molen & Stella M. K. Glasauer & Elmer Guzman & Alessio P. Buccino & Gabriel Luna & Zhuowei Cheng & Morgane Audouard & Kamalini G. Ranasinghe & Kiwamu Kudo & Srikantan S. Nagarajan &, 2022. "Functional neuronal circuitry and oscillatory dynamics in human brain organoids," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    5. Carl E. Schoonover & Sarah N. Ohashi & Richard Axel & Andrew J. P. Fink, 2021. "Representational drift in primary olfactory cortex," Nature, Nature, vol. 594(7864), pages 541-546, June.
    6. Madeline A. Lancaster & Magdalena Renner & Carol-Anne Martin & Daniel Wenzel & Louise S. Bicknell & Matthew E. Hurles & Tessa Homfray & Josef M. Penninger & Andrew P. Jackson & Juergen A. Knoblich, 2013. "Cerebral organoids model human brain development and microcephaly," Nature, Nature, vol. 501(7467), pages 373-379, September.
    7. Shan Jiang & Dipan C. Patel & Jongwoon Kim & Shuo Yang & William A. Mills & Yujing Zhang & Kaiwen Wang & Ziang Feng & Sujith Vijayan & Wenjun Cai & Anbo Wang & Yuanyuan Guo & Ian F. Kimbrough & Harald, 2020. "Spatially expandable fiber-based probes as a multifunctional deep brain interface," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
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