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Quantitative determination of the spatial distribution of components in single cells with CellDetail

Author

Listed:
  • Tanja Schuster

    (Ulm University)

  • Amanda Amoah

    (Ulm University
    BC Cancer Research Centre)

  • Angelika Vollmer

    (Ulm University)

  • Gina Marka

    (Ulm University)

  • Julian Niemann

    (Ulm University)

  • Mehmet Saçma

    (Ulm University)

  • Vadim Sakk

    (Ulm University)

  • Karin Soller

    (Ulm University)

  • Mona Vogel

    (Ulm University)

  • Ani Grigoryan

    (Ulm University)

  • Meinhard Wlaschek

    (Ulm University)

  • Karin Scharffetter-Kochanek

    (Ulm University)

  • Medhanie Mulaw

    (Ulm University)

  • Hartmut Geiger

    (Ulm University)

Abstract

The distribution of biomolecules within cells changes upon aging and diseases. To quantitatively determine the spatial distribution of components inside cells, we built the user-friendly open-source 3D-cell-image analysis platform Cell Detection and Analysis of Intensity Lounge (CellDetail). The algorithm within CellDetail is based on the concept of the dipole moment. CellDetail provides quantitative values for the distribution of the polarity proteins Cdc42 and Tubulin in young and aged hematopoietic stem cells (HSCs). Septin proteins form networks within cells that are critical for cell compartmentalization. We uncover a reduced level of organization of the Septin network within aged HSCs and within senescent human fibroblasts. Changes in the Septin network structure might therefore be a common feature of aging. The level of organization of the network of Septin proteins in aged HSCs can be restored to a youthful level by pharmacological attenuation of the activity of the small RhoGTPase Cdc42.

Suggested Citation

  • Tanja Schuster & Amanda Amoah & Angelika Vollmer & Gina Marka & Julian Niemann & Mehmet Saçma & Vadim Sakk & Karin Soller & Mona Vogel & Ani Grigoryan & Meinhard Wlaschek & Karin Scharffetter-Kochanek, 2024. "Quantitative determination of the spatial distribution of components in single cells with CellDetail," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54638-8
    DOI: 10.1038/s41467-024-54638-8
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    References listed on IDEAS

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