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Long-term machine perfusion of human split livers: a new model for regenerative and translational research

Author

Listed:
  • Ngee-Soon Lau

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Geoffrey McCaughan

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Mark Ly

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Ken Liu

    (Royal Prince Alfred Hospital
    The University of Sydney)

  • Michael Crawford

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Carlo Pulitano

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

Abstract

Recent advances in machine perfusion have revolutionised the field of transplantation by prolonging preservation, permitting evaluation of viability prior to implant and rescue of discarded organs. Long-term perfusion for days-to-weeks provides time to modify these organs prior to transplantation. By using long-term normothermic machine perfusion to facilitate liver splitting and subsequent perfusion of both partial organs, possibilities even outside the clinical arena become possible. This model remains in its infancy but in the future, could allow for detailed study of liver injury and regeneration, and ex-situ treatment strategies such as defatting, genetic modulation and stem-cell therapies. Here we provide insight into this new model for research and highlight its great potential and current limitations.

Suggested Citation

  • Ngee-Soon Lau & Geoffrey McCaughan & Mark Ly & Ken Liu & Michael Crawford & Carlo Pulitano, 2024. "Long-term machine perfusion of human split livers: a new model for regenerative and translational research," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54024-4
    DOI: 10.1038/s41467-024-54024-4
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    References listed on IDEAS

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    1. Claudia Y. Janda & Luke T. Dang & Changjiang You & Junlei Chang & Wim de Lau & Zhendong A. Zhong & Kelley S. Yan & Owen Marecic & Dirk Siepe & Xingnan Li & James D. Moody & Bart O. Williams & Hans Cle, 2017. "Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling," Nature, Nature, vol. 545(7653), pages 234-237, May.
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    3. Hynek Mergental & Richard W. Laing & Amanda J. Kirkham & M. Thamara P. R. Perera & Yuri L. Boteon & Joseph Attard & Darren Barton & Stuart Curbishley & Manpreet Wilkhu & Desley A. H. Neil & Stefan G. , 2020. "Transplantation of discarded livers following viability testing with normothermic machine perfusion," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    4. Ngee-Soon Lau & Mark Ly & Claude Dennis & Andrew Jacques & Marti Cabanes-Creus & Shamus Toomath & Joanna Huang & Nicole Mestrovic & Paul Yousif & Sumon Chanda & Chuanmin Wang & Leszek Lisowski & Ken L, 2023. "Long-term ex situ normothermic perfusion of human split livers for more than 1 week," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    5. Marti Cabanes-Creus & Sophia H. Y. Liao & Renina Gale Navarro & Maddison Knight & Deborah Nazareth & Ngee-Soon Lau & Mark Ly & Erhua Zhu & Ramon Roca-Pinilla & Ricardo Bugallo Delgado & Ana F. Vicente, 2024. "Harnessing whole human liver ex situ normothermic perfusion for preclinical AAV vector evaluation," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
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