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Long-term ex situ normothermic perfusion of human split livers for more than 1 week

Author

Listed:
  • Ngee-Soon Lau

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Mark Ly

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Claude Dennis

    (Royal Prince Alfred Hospital)

  • Andrew Jacques

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Marti Cabanes-Creus

    (The University of Sydney, Westmead)

  • Shamus Toomath

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital)

  • Joanna Huang

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Nicole Mestrovic

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Paul Yousif

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital)

  • Sumon Chanda

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital)

  • Chuanmin Wang

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Leszek Lisowski

    (The University of Sydney, Westmead
    Laboratory of Molecular Oncology and Innovative Therapies
    Children’s Medical Research Institute and Sydney Children’s Hospitals Network)

  • Ken Liu

    (Royal Prince Alfred Hospital
    The University of Sydney)

  • James G. Kench

    (The University of Sydney
    Royal Prince Alfred Hospital)

  • Geoffrey McCaughan

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney
    Centenary Institute)

  • Michael Crawford

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

  • Carlo Pulitano

    (Royal Prince Alfred Hospital
    Royal Prince Alfred Hospital
    The University of Sydney)

Abstract

Current machine perfusion technology permits livers to be preserved ex situ for short periods to assess viability prior to transplant. Long-term normothermic perfusion of livers is an emerging field with tremendous potential for the assessment, recovery, and modification of organs. In this study, we aimed to develop a long-term model of ex situ perfusion including a surgical split and simultaneous perfusion of both partial organs. Human livers declined for transplantation were perfused using a red blood cell-based perfusate under normothermic conditions (36 °C) and then split and simultaneously perfused on separate machines. Ten human livers were split, resulting in 20 partial livers. The median ex situ viability was 125 h, and the median ex situ survival was 165 h. Long-term survival was demonstrated by lactate clearance, bile production, Factor-V production, and storage of adenosine triphosphate. Here, we report the long-term ex situ perfusion of human livers and demonstrate the ability to split and perfuse these organs using a standardised protocol.

Suggested Citation

  • Ngee-Soon Lau & Mark Ly & Claude Dennis & Andrew Jacques & Marti Cabanes-Creus & Shamus Toomath & Joanna Huang & Nicole Mestrovic & Paul Yousif & Sumon Chanda & Chuanmin Wang & Leszek Lisowski & Ken L, 2023. "Long-term ex situ normothermic perfusion of human split livers for more than 1 week," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40154-8
    DOI: 10.1038/s41467-023-40154-8
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    Cited by:

    1. Ngee-Soon Lau & Geoffrey McCaughan & Mark Ly & Ken Liu & Michael Crawford & Carlo Pulitano, 2024. "Long-term machine perfusion of human split livers: a new model for regenerative and translational research," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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