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Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

Author

Listed:
  • Ohman Kwon

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Hana Lee

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Jaeeun Jung

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Ye Seul Son

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Sojeong Jeon

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Won Dong Yoo

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    Korea University of Science and Technology (UST))

  • Naeun Son

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    Korea University of Science and Technology (UST))

  • Kwang Bo Jung

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Eunho Choi

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    Korea University of Science and Technology (UST))

  • In-Chul Lee

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    KRIBB, Korea Preclinical Evaluation Center)

  • Hyung-Jun Kwon

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    KRIBB, Korea Preclinical Evaluation Center)

  • Chuna Kim

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    Korea University of Science and Technology (UST)
    KRIBB, Aging Convergence Research Center)

  • Mi-Ok Lee

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    Korea University of Science and Technology (UST))

  • Hyun-Soo Cho

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    Korea University of Science and Technology (UST)
    Sungkyunkwan University)

  • Dae Soo Kim

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    Korea University of Science and Technology (UST))

  • Mi-Young Son

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB)
    Korea University of Science and Technology (UST)
    Sungkyunkwan University)

Abstract

Three-dimensional human intestinal organoids (hIO) are widely used as a platform for biological and biomedical research. However, reproducibility and challenges for large-scale expansion limit their applicability. Here, we establish a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC3D-hIO) within hIO derived from human pluripotent stem cells. The intrinsic self-organisation property of ISC3D-hIO, combined with air-liquid interface culture in a minimally defined medium, forces ISC3D-hIO to differentiate into the intestinal epithelium with cellular diversity, villus-like structure, and barrier integrity. Notably, ISC3D-hIO is an ideal cell source for gene editing to study ISC biology and transplantation for intestinal diseases. We demonstrate the intestinal epithelium differentiated from ISC3D-hIO as a model system to study severe acute respiratory syndrome coronavirus 2 viral infection. ISC3D-hIO culture technology provides a biological tool for use in regenerative medicine and disease modelling.

Suggested Citation

  • Ohman Kwon & Hana Lee & Jaeeun Jung & Ye Seul Son & Sojeong Jeon & Won Dong Yoo & Naeun Son & Kwang Bo Jung & Eunho Choi & In-Chul Lee & Hyung-Jun Kwon & Chuna Kim & Mi-Ok Lee & Hyun-Soo Cho & Dae Soo, 2024. "Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoids," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45103-7
    DOI: 10.1038/s41467-024-45103-7
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    References listed on IDEAS

    as
    1. Kwang Bo Jung & Hana Lee & Ye Seul Son & Mi-Ok Lee & Young-Dae Kim & Soo Jin Oh & Ohman Kwon & Sunwha Cho & Hyun-Soo Cho & Dae-Soo Kim & Jung-Hwa Oh & Matthias Zilbauer & Jeong-Ki Min & Cho-Rok Jung &, 2018. "Interleukin-2 induces the in vitro maturation of human pluripotent stem cell-derived intestinal organoids," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
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