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In vivo imaging of vagal-induced myenteric plexus responses in gastrointestinal tract with an optical window

Author

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  • Longjie Jiang

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Jie Yang

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Xiujuan Gao

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Jiangfeng Huang

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Qian Liu

    (Hainan University)

  • Ling Fu

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology
    Hainan University
    Hainan University)

Abstract

The vagus nerve (VN) extensively innervates the gastric enteric nervous system (ENS), but its influence on gastric ENS functionality and motility in vivo remains unclear due to technical challenges. Here we describe a method for stable, long-term observation of gastric ENS activity and muscle dynamics at cellular resolution, which can also be extended to intestinal applications. This method involves ENS-specific labeling and the implantation of an abdominal wall window for optical recording in male mice. In vivo calcium imaging reveals a linear relationship between vagal stimulation frequency and myenteric neuron activation in gastric antrum. Furthermore, the motility of gastric antrum is significantly enhanced and shows a positive correlation with the intensity and number of activated myenteric neurons. While vagal stimulation also activates proximal colonic myenteric neurons, this activation is not frequency-dependent and does not induce proximal colonic motility. The method and results provide important insights into VN-ENS interactions in vivo, advancing our understanding of gastrointestinal motility regulation.

Suggested Citation

  • Longjie Jiang & Jie Yang & Xiujuan Gao & Jiangfeng Huang & Qian Liu & Ling Fu, 2024. "In vivo imaging of vagal-induced myenteric plexus responses in gastrointestinal tract with an optical window," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52397-0
    DOI: 10.1038/s41467-024-52397-0
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