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Long-term imaging of dorsal root ganglia in awake behaving mice

Author

Listed:
  • Chao Chen

    (Peking 301 Hospital)

  • Jinhui Zhang

    (the Affiliated Southeast Hospital of Xiamen University)

  • Linlin Sun

    (Columbia University)

  • Yiling Zhang

    (Peking 301 Hospital)

  • Wen-Biao Gan

    (New York University School of Medicine)

  • Peifu Tang

    (Peking 301 Hospital)

  • Guang Yang

    (Columbia University)

Abstract

The dorsal root ganglia (DRG) contain the somas of first-order sensory neurons critical for somatosensation. Due to technical difficulties, DRG neuronal activity in awake behaving animals remains unknown. Here, we develop a method for imaging DRG at cellular and subcellular resolution over weeks in awake mice. The method involves the installation of an intervertebral fusion mount to reduce spinal movement, and the implantation of a vertebral glass window without interfering animals’ motor and sensory functions. In vivo two-photon calcium imaging shows that DRG neuronal activity is higher in awake than anesthetized animals. Immediately after plantar formalin injection, DRG neuronal activity increases substantially and this activity upsurge correlates with animals’ phasic pain behavior. Repeated imaging of DRG over 5 weeks after formalin injection reveals persistent neuronal hyperactivity associated with ongoing pain. The method described here provides an important means for in vivo studies of DRG functions in sensory perception and disorders.

Suggested Citation

  • Chao Chen & Jinhui Zhang & Linlin Sun & Yiling Zhang & Wen-Biao Gan & Peifu Tang & Guang Yang, 2019. "Long-term imaging of dorsal root ganglia in awake behaving mice," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11158-0
    DOI: 10.1038/s41467-019-11158-0
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    Cited by:

    1. Longjie Jiang & Jie Yang & Xiujuan Gao & Jiangfeng Huang & Qian Liu & Ling Fu, 2024. "In vivo imaging of vagal-induced myenteric plexus responses in gastrointestinal tract with an optical window," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Chao Chen & Linlin Sun & Avital Adler & Hang Zhou & Licheng Zhang & Lihai Zhang & Junhao Deng & Yang Bai & Jinhui Zhang & Guang Yang & Wen-Biao Gan & Peifu Tang, 2023. "Synchronized activity of sensory neurons initiates cortical synchrony in a model of neuropathic pain," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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