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Synergistic collaboration between AMPs and non-direct antimicrobial cationic peptides

Author

Listed:
  • Zifan Ye

    (Soochow University)

  • Lei Fu

    (Beijing Normal University)

  • Shuangyu Li

    (Soochow University)

  • Ziying Chen

    (Beijing Normal University)

  • Jianhong Ouyang

    (Soochow University)

  • Xinci Shang

    (Soochow University)

  • Yanli Liu

    (Soochow University)

  • Lianghui Gao

    (Beijing Normal University)

  • Yipeng Wang

    (Soochow University
    Chinese Academy of Sciences)

Abstract

Non-direct antimicrobial cationic peptides (NDACPs) are components of the animal innate immune system. But their functions and association with antimicrobial peptides (AMPs) are incompletely understood. Here, we reveal a synergistic interaction between the AMP AW1 and the NDACP AW2, which are co-expressed in the frog Amolops wuyiensis. AW2 enhances the antibacterial activity of AW1 both in vitro and in vivo, while mitigating the development of bacterial resistance and eradicating biofilms. AW1 and AW2 synergistically damage bacterial membranes, facilitating cellular uptake and interaction of AW2 with the intracellular target bacterial genomic DNA. Simultaneously, they trigger the generation of ROS in bacteria, contributing to cell death upon reaching a threshold level. Moreover, we demonstrate that this synergistic antibacterial effect between AMPs and NDACPs is prevalent across diverse animal species. These findings unveil a robust and previously unknown correlation between AMPs and NDACPs as a widespread antibacterial immune defense strategy in animals.

Suggested Citation

  • Zifan Ye & Lei Fu & Shuangyu Li & Ziying Chen & Jianhong Ouyang & Xinci Shang & Yanli Liu & Lianghui Gao & Yipeng Wang, 2024. "Synergistic collaboration between AMPs and non-direct antimicrobial cationic peptides," Nature Communications, Nature, vol. 15(1), pages 1-26, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51730-x
    DOI: 10.1038/s41467-024-51730-x
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    References listed on IDEAS

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