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Nanopore ion sources deliver individual ions of amino acids and peptides directly into high vacuum

Author

Listed:
  • Nicholas Drachman

    (Brown University)

  • Mathilde Lepoitevin

    (Brown University)

  • Hannah Szapary

    (Brown University)

  • Benjamin Wiener

    (Brown University)

  • William Maulbetsch

    (Brown University)

  • Derek Stein

    (Brown University
    Brown University)

Abstract

Electrospray ionization is widely used to generate vapor phase ions for analysis by mass spectrometry in proteomics research. However, only a small fraction of the analyte enters the mass spectrometer due to losses that are fundamentally linked to the use of a background gas to stimulate the generation of ions from electrosprayed droplets. Here we report a nanopore ion source that delivers ions directly into high vacuum from aqueous solutions. The ion source comprises a pulled quartz pipette with a sub-100 nm opening. Ions escape an electrified meniscus by ion evaporation and travel along collisionless trajectories to the ion detector. We measure mass spectra of 16 different amino acid ions, post-translationally modified variants of glutathione, and the peptide angiotensin II, showing that these analytes can be emitted as desolvated ions. The emitted current is composed of ions rather than charged droplets, and more than 90% of the current can be recovered in a distant collector.

Suggested Citation

  • Nicholas Drachman & Mathilde Lepoitevin & Hannah Szapary & Benjamin Wiener & William Maulbetsch & Derek Stein, 2024. "Nanopore ion sources deliver individual ions of amino acids and peptides directly into high vacuum," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51455-x
    DOI: 10.1038/s41467-024-51455-x
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    References listed on IDEAS

    as
    1. Ruedi Aebersold & Matthias Mann, 2016. "Mass-spectrometric exploration of proteome structure and function," Nature, Nature, vol. 537(7620), pages 347-355, September.
    2. J. A. Sellberg & C. Huang & T. A. McQueen & N. D. Loh & H. Laksmono & D. Schlesinger & R. G. Sierra & D. Nordlund & C. Y. Hampton & D. Starodub & D. P. DePonte & M. Beye & C. Chen & A. V. Martin & A. , 2014. "Ultrafast X-ray probing of water structure below the homogeneous ice nucleation temperature," Nature, Nature, vol. 510(7505), pages 381-384, June.
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