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Cell-selective proteomics reveal novel effectors secreted by an obligate intracellular bacterial pathogen

Author

Listed:
  • Allen G. Sanderlin

    (Massachusetts Institute of Technology)

  • Hannah Kurka Margolis

    (Massachusetts Institute of Technology)

  • Abigail F. Meyer

    (Massachusetts Institute of Technology)

  • Rebecca L. Lamason

    (Massachusetts Institute of Technology)

Abstract

Pathogenic bacteria secrete protein effectors to hijack host machinery and remodel their infectious niche. Rickettsia spp. are obligate intracellular bacteria that can cause life-threatening disease, but their absolute dependence on the host cell has impeded discovery of rickettsial effectors and their host targets. We implemented bioorthogonal non-canonical amino acid tagging (BONCAT) during R. parkeri infection to selectively label, isolate, and identify effectors delivered into the host cell. As the first use of BONCAT in an obligate intracellular bacterium, our screen more than doubles the number of experimentally validated effectors for the genus. The seven novel secreted rickettsial factors (Srfs) we identified include Rickettsia-specific proteins of unknown function that localize to the host cytoplasm, mitochondria, and ER. We further show that one such effector, SrfD, interacts with the host Sec61 translocon. Altogether, our work uncovers a diverse set of previously uncharacterized rickettsial effectors and lays the foundation for a deeper exploration of the host-pathogen interface.

Suggested Citation

  • Allen G. Sanderlin & Hannah Kurka Margolis & Abigail F. Meyer & Rebecca L. Lamason, 2024. "Cell-selective proteomics reveal novel effectors secreted by an obligate intracellular bacterial pathogen," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50493-9
    DOI: 10.1038/s41467-024-50493-9
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    References listed on IDEAS

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    1. Lea Krampen & Silke Malmsheimer & Iwan Grin & Thomas Trunk & Anja Lührmann & Jan-Willem Gier & Samuel Wagner, 2018. "Revealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    2. Siv G. E. Andersson & Alireza Zomorodipour & Jan O. Andersson & Thomas Sicheritz-Pontén & U. Cecilia M. Alsmark & Raf M. Podowski & A. Kristina Näslund & Ann-Sofie Eriksson & Herbert H. Winkler & Char, 1998. "The genome sequence of Rickettsia prowazekii and the origin of mitochondria," Nature, Nature, vol. 396(6707), pages 133-140, November.
    3. Gina M. Borgo & Thomas P. Burke & Cuong J. Tran & Nicholas T. N. Lo & Patrik Engström & Matthew D. Welch, 2022. "A patatin-like phospholipase mediates Rickettsia parkeri escape from host membranes," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    4. Bethany A. Weigele & Robert C. Orchard & Alyssa Jimenez & Gregory W. Cox & Neal M. Alto, 2017. "A systematic exploration of the interactions between bacterial effector proteins and host cell membranes," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
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