IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-00700-7.html
   My bibliography  Save this article

A systematic exploration of the interactions between bacterial effector proteins and host cell membranes

Author

Listed:
  • Bethany A. Weigele

    (University of Texas Southwestern Medical Center)

  • Robert C. Orchard

    (Washington University School of Medicine)

  • Alyssa Jimenez

    (University of Texas Southwestern Medical Center)

  • Gregory W. Cox

    (University of Texas Southwestern Medical Center)

  • Neal M. Alto

    (University of Texas Southwestern Medical Center)

Abstract

Membrane-bound organelles serve as platforms for the assembly of multi-protein complexes that function as hubs of signal transduction in eukaryotic cells. Microbial pathogens have evolved virulence factors that reprogram these host signaling responses, but the underlying molecular mechanisms are poorly understood. Here we test the ability of ~200 type III and type IV effector proteins from six Gram-negative bacterial species to interact with the eukaryotic plasma membrane and intracellular organelles. We show that over 30% of the effectors localize to yeast and mammalian cell membranes, including a subset of previously uncharacterized Legionella effectors that appear to be able to regulate yeast vacuolar fusion. A combined genetic, cellular, and biochemical approach supports that some of the tested bacterial effectors can bind to membrane phospholipids and may regulate membrane trafficking. Finally, we show that the type III effector IpgB1 from Shigella flexneri may bind to acidic phospholipids and regulate actin filament dynamics.

Suggested Citation

  • Bethany A. Weigele & Robert C. Orchard & Alyssa Jimenez & Gregory W. Cox & Neal M. Alto, 2017. "A systematic exploration of the interactions between bacterial effector proteins and host cell membranes," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00700-7
    DOI: 10.1038/s41467-017-00700-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-00700-7
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-017-00700-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Allen G. Sanderlin & Hannah Kurka Margolis & Abigail F. Meyer & Rebecca L. Lamason, 2024. "Cell-selective proteomics reveal novel effectors secreted by an obligate intracellular bacterial pathogen," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00700-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.