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Revealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems

Author

Listed:
  • Lea Krampen

    (Interfaculty Institute of Microbiology and Infection Medicine (IMIT))

  • Silke Malmsheimer

    (Interfaculty Institute of Microbiology and Infection Medicine (IMIT))

  • Iwan Grin

    (Interfaculty Institute of Microbiology and Infection Medicine (IMIT))

  • Thomas Trunk

    (Interfaculty Institute of Microbiology and Infection Medicine (IMIT)
    University of Oslo)

  • Anja Lührmann

    (University Hospital Erlangen)

  • Jan-Willem Gier

    (Stockholm University)

  • Samuel Wagner

    (Interfaculty Institute of Microbiology and Infection Medicine (IMIT)
    Partner-site Tübingen)

Abstract

Many bacteria export effector proteins fulfilling their function in membranes of a eukaryotic host. These effector membrane proteins appear to contain signals for two incompatible bacterial secretion pathways in the same protein: a specific export signal, as well as transmembrane segments that one would expect to mediate targeting to the bacterial inner membrane. Here, we show that the transmembrane segments of effector proteins of type III and type IV secretion systems indeed integrate in the membrane as required in the eukaryotic host, but that their hydrophobicity in most instances is just below the threshold required for mediating targeting to the bacterial inner membrane. Furthermore, we show that binding of type III secretion chaperones to both the effector’s chaperone-binding domain and adjacent hydrophobic transmembrane segments also prevents erroneous targeting. These results highlight the evolution of a fine discrimination between targeting pathways that is critical for the virulence of many bacterial pathogens.

Suggested Citation

  • Lea Krampen & Silke Malmsheimer & Iwan Grin & Thomas Trunk & Anja Lührmann & Jan-Willem Gier & Samuel Wagner, 2018. "Revealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05969-w
    DOI: 10.1038/s41467-018-05969-w
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    Cited by:

    1. Allen G. Sanderlin & Hannah Kurka Margolis & Abigail F. Meyer & Rebecca L. Lamason, 2024. "Cell-selective proteomics reveal novel effectors secreted by an obligate intracellular bacterial pathogen," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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