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A quantitative gibberellin signaling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem

Author

Listed:
  • Bihai Shi

    (South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture
    Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA)

  • Amelia Felipo-Benavent

    (CNRS, Université de Strasbourg)

  • Guillaume Cerutti

    (Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA)

  • Carlos Galvan-Ampudia

    (Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA)

  • Lucas Jilli

    (CNRS, Université de Strasbourg)

  • Geraldine Brunoud

    (Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA)

  • Jérome Mutterer

    (CNRS, Université de Strasbourg)

  • Elody Vallet

    (CNRS, Université de Strasbourg)

  • Lali Sakvarelidze-Achard

    (CNRS, Université de Strasbourg)

  • Jean-Michel Davière

    (CNRS, Université de Strasbourg)

  • Alejandro Navarro-Galiano

    (08193 Cerdanyola)

  • Ankit Walia

    (Cambridge University)

  • Shani Lazary

    (Tel Aviv University)

  • Jonathan Legrand

    (Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA)

  • Roy Weinstain

    (Tel Aviv University)

  • Alexander M. Jones

    (Cambridge University)

  • Salomé Prat

    (08193 Cerdanyola)

  • Patrick Achard

    (CNRS, Université de Strasbourg)

  • Teva Vernoux

    (Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA)

Abstract

Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signaling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signaling activity in the SAM. We show that high GA signaling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organization of internodes, thus contributing to internode specification in the SAM.

Suggested Citation

  • Bihai Shi & Amelia Felipo-Benavent & Guillaume Cerutti & Carlos Galvan-Ampudia & Lucas Jilli & Geraldine Brunoud & Jérome Mutterer & Elody Vallet & Lali Sakvarelidze-Achard & Jean-Michel Davière & Ale, 2024. "A quantitative gibberellin signaling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48116-4
    DOI: 10.1038/s41467-024-48116-4
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    References listed on IDEAS

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