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The Arabidopsis NPF3 protein is a GA transporter

Author

Listed:
  • Iris Tal

    (Tel Aviv University)

  • Yi Zhang

    (Howard Hughes Medical Institute and Section of Cell and Developmental Biology, University of California San Diego)

  • Morten Egevang Jørgensen

    (DynaMo Center, Faculty of Science, University of Copenhagen)

  • Odelia Pisanty

    (Tel Aviv University)

  • Inês C. R. Barbosa

    (Plant Systems Biology, Technische Universität München)

  • Melina Zourelidou

    (Plant Systems Biology, Technische Universität München)

  • Thomas Regnault

    (Plant Systems Biology, Technische Universität München)

  • Christoph Crocoll

    (DynaMo Center, Faculty of Science, University of Copenhagen)

  • Carl Erik Olsen

    (Faculty of Science, University of Copenhagen)

  • Roy Weinstain

    (Tel Aviv University)

  • Claus Schwechheimer

    (Plant Systems Biology, Technische Universität München)

  • Barbara Ann Halkier

    (DynaMo Center, Faculty of Science, University of Copenhagen)

  • Hussam Hassan Nour-Eldin

    (DynaMo Center, Faculty of Science, University of Copenhagen)

  • Mark Estelle

    (Howard Hughes Medical Institute and Section of Cell and Developmental Biology, University of California San Diego)

  • Eilon Shani

    (Tel Aviv University)

Abstract

Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA–ABA interaction may occur at the level of transport.

Suggested Citation

  • Iris Tal & Yi Zhang & Morten Egevang Jørgensen & Odelia Pisanty & Inês C. R. Barbosa & Melina Zourelidou & Thomas Regnault & Christoph Crocoll & Carl Erik Olsen & Roy Weinstain & Claus Schwechheimer &, 2016. "The Arabidopsis NPF3 protein is a GA transporter," Nature Communications, Nature, vol. 7(1), pages 1-11, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11486
    DOI: 10.1038/ncomms11486
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    Cited by:

    1. Bihai Shi & Amelia Felipo-Benavent & Guillaume Cerutti & Carlos Galvan-Ampudia & Lucas Jilli & Geraldine Brunoud & Jérome Mutterer & Elody Vallet & Lali Sakvarelidze-Achard & Jean-Michel Davière & Ale, 2024. "A quantitative gibberellin signaling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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