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Structure of the human Bre1 complex bound to the nucleosome

Author

Listed:
  • Shuhei Onishi

    (Yokohama City University Graduate School of Medicine)

  • Kotone Uchiyama

    (Yokohama City University Graduate School of Medicine)

  • Ko Sato

    (Yokohama City University Graduate School of Medicine)

  • Chikako Okada

    (Yokohama City University Graduate School of Medicine)

  • Shunsuke Kobayashi

    (Yokohama City University Graduate School of Medicine)

  • Keisuke Hamada

    (Yokohama City University Graduate School of Medicine)

  • Tomohiro Nishizawa

    (The University of Tokyo
    Yokohama City University)

  • Osamu Nureki

    (The University of Tokyo)

  • Kazuhiro Ogata

    (Yokohama City University Graduate School of Medicine)

  • Toru Sengoku

    (Yokohama City University Graduate School of Medicine)

Abstract

Histone H2B monoubiquitination (at Lys120 in humans) regulates transcription elongation and DNA repair. In humans, H2B monoubiquitination is catalyzed by the heterodimeric Bre1 complex composed of Bre1A/RNF20 and Bre1B/RNF40. The Bre1 proteins generally function as tumor suppressors, while in certain cancers, they facilitate cancer cell proliferation. To obtain structural insights of H2BK120 ubiquitination and its regulation, we report the cryo-electron microscopy structure of the human Bre1 complex bound to the nucleosome. The two RING domains of Bre1A and Bre1B recognize the acidic patch and the nucleosomal DNA phosphates around SHL 6.0–6.5, which are ideally located to recruit the E2 enzyme and ubiquitin for H2BK120-specific ubiquitination. Mutational experiments suggest that the two RING domains bind in two orientations and that ubiquitination occurs when Bre1A binds to the acidic patch. Our results provide insights into the H2BK120-specific ubiquitination by the Bre1 proteins and suggest that H2B monoubiquitination can be regulated by nuclesomal DNA flexibility.

Suggested Citation

  • Shuhei Onishi & Kotone Uchiyama & Ko Sato & Chikako Okada & Shunsuke Kobayashi & Keisuke Hamada & Tomohiro Nishizawa & Osamu Nureki & Kazuhiro Ogata & Toru Sengoku, 2024. "Structure of the human Bre1 complex bound to the nucleosome," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46910-8
    DOI: 10.1038/s41467-024-46910-8
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    References listed on IDEAS

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