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FLIP(C1orf112)-FIGNL1 complex regulates RAD51 chromatin association to promote viability after replication stress

Author

Listed:
  • Jessica D. Tischler

    (Fred Hutchinson Cancer Center)

  • Hiroshi Tsuchida

    (Fred Hutchinson Cancer Center)

  • Rosevalentine Bosire

    (Fred Hutchinson Cancer Center)

  • Tommy T. Oda

    (Fred Hutchinson Cancer Center
    University of Washington)

  • Ana Park

    (Fred Hutchinson Cancer Center
    University of Washington)

  • Richard O. Adeyemi

    (Fred Hutchinson Cancer Center)

Abstract

Homologous recombination (HR) plays critical roles in repairing lesions that arise during DNA replication and is thus essential for viability. RAD51 plays important roles during replication and HR, however, how RAD51 is regulated downstream of nucleofilament formation and how the varied RAD51 functions are regulated is not clear. We have investigated the protein c1orf112/FLIP that previously scored in genome-wide screens for mediators of DNA inter-strand crosslink (ICL) repair. Upon ICL agent exposure, FLIP loss leads to marked cell death, elevated chromosomal instability, increased micronuclei formation, altered cell cycle progression and increased DNA damage signaling. FLIP is recruited to damage foci and forms a complex with FIGNL1. Both proteins have epistatic roles in ICL repair, forming a stable complex. Mechanistically, FLIP loss leads to increased RAD51 amounts and foci on chromatin both with or without exogenous DNA damage, defective replication fork progression and reduced HR competency. We posit that FLIP is essential for limiting RAD51 levels on chromatin in the absence of damage and for RAD51 dissociation from nucleofilaments to properly complete HR. Failure to do so leads to replication slowing and inability to complete repair.

Suggested Citation

  • Jessica D. Tischler & Hiroshi Tsuchida & Rosevalentine Bosire & Tommy T. Oda & Ana Park & Richard O. Adeyemi, 2024. "FLIP(C1orf112)-FIGNL1 complex regulates RAD51 chromatin association to promote viability after replication stress," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45139-9
    DOI: 10.1038/s41467-024-45139-9
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    1. Akbar Zainu & Pauline Dupaigne & Soumya Bouchouika & Julien Cau & Julie A. J. Clément & Pauline Auffret & Virginie Ropars & Jean-Baptiste Charbonnier & Bernard Massy & Raphael Mercier & Rajeev Kumar &, 2024. "FIGNL1-FIRRM is essential for meiotic recombination and prevents DNA damage-independent RAD51 and DMC1 loading," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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