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Integrative single-cell characterization of a frugivorous and an insectivorous bat kidney and pancreas

Author

Listed:
  • Wei E. Gordon

    (University of California, San Francisco
    University of California, San Francisco
    Menlo College)

  • Seungbyn Baek

    (Yonsei University)

  • Hai P. Nguyen

    (University of California, San Francisco
    University of California, San Francisco)

  • Yien-Ming Kuo

    (University of California, San Francisco)

  • Rachael Bradley

    (University of California, San Francisco
    University of California, San Francisco)

  • Sarah L. Fong

    (University of California, San Francisco
    University of California, San Francisco)

  • Nayeon Kim

    (Yonsei University)

  • Alex Galazyuk

    (Northeast Ohio Medical University)

  • Insuk Lee

    (Yonsei University
    Pohang University of Science and Technology (POSTECH))

  • Melissa R. Ingala

    (Fairleigh Dickinson University)

  • Nancy B. Simmons

    (American Museum of Natural History)

  • Tony Schountz

    (Colorado State University)

  • Lisa Noelle Cooper

    (Northeast Ohio Medical University)

  • Ilias Georgakopoulos-Soares

    (The Pennsylvania State University College of Medicine)

  • Martin Hemberg

    (Brigham and Women’s Hospital and Harvard Medical School)

  • Nadav Ahituv

    (University of California, San Francisco
    University of California, San Francisco)

Abstract

Frugivory evolved multiple times in mammals, including bats. However, the cellular and molecular components driving it remain largely unknown. Here, we use integrative single-cell sequencing (scRNA-seq and scATAC-seq) on insectivorous (Eptesicus fuscus; big brown bat) and frugivorous (Artibeus jamaicensis; Jamaican fruit bat) bat kidneys and pancreases and identify key cell population, gene expression and regulatory differences associated with the Jamaican fruit bat that also relate to human disease, particularly diabetes. We find a decrease in loop of Henle and an increase in collecting duct cells, and differentially active genes and regulatory elements involved in fluid and electrolyte balance in the Jamaican fruit bat kidney. The Jamaican fruit bat pancreas shows an increase in endocrine and a decrease in exocrine cells, and differences in genes and regulatory elements involved in insulin regulation. We also find that these frugivorous bats share several molecular characteristics with human diabetes. Combined, our work provides insights from a frugivorous mammal that could be leveraged for therapeutic purposes.

Suggested Citation

  • Wei E. Gordon & Seungbyn Baek & Hai P. Nguyen & Yien-Ming Kuo & Rachael Bradley & Sarah L. Fong & Nayeon Kim & Alex Galazyuk & Insuk Lee & Melissa R. Ingala & Nancy B. Simmons & Tony Schountz & Lisa N, 2024. "Integrative single-cell characterization of a frugivorous and an insectivorous bat kidney and pancreas," Nature Communications, Nature, vol. 15(1), pages 1-23, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44186-y
    DOI: 10.1038/s41467-023-44186-y
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    References listed on IDEAS

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