IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-44000-9.html
   My bibliography  Save this article

Functional and analytical recapitulation of osteoclast biology on demineralized bone paper

Author

Listed:
  • Yongkuk Park

    (University of Massachusetts)

  • Tadatoshi Sato

    (UMass Chan Medical School)

  • Jungwoo Lee

    (University of Massachusetts
    University of Massachusetts
    University of Massachusetts)

Abstract

Osteoclasts are the primary target for osteoporosis drug development. Recent animal studies revealed the crucial roles of osteoblasts in regulating osteoclastogenesis and the longer lifespans of osteoclasts than previously thought with fission and recycling. However, existing culture platforms are limited to replicating these newly identified cellular processes. We report a demineralized bone paper (DBP)-based osteoblast culture and osteoclast assay platform that replicates osteoclast fusion, fission, resorption, and apoptosis with high fidelity and analytical power. An osteoid-inspired DBP supports rapid and structural mineral deposition by osteoblasts. Coculture osteoblasts and bone marrow monocytes under biochemical stimulation recapitulate osteoclast differentiation and function. The DBP-based bone model allows longitudinal quantitative fluorescent monitoring of osteoclast responses to bisphosphonate drug, substantiating significantly reducing their number and lifespan. Finally, we demonstrate the feasibility of humanizing the bone model. The DBP-based osteo assay platforms are expected to advance bone remodeling-targeting drug development with improved prediction of clinical outcomes.

Suggested Citation

  • Yongkuk Park & Tadatoshi Sato & Jungwoo Lee, 2023. "Functional and analytical recapitulation of osteoclast biology on demineralized bone paper," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-44000-9
    DOI: 10.1038/s41467-023-44000-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-44000-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-44000-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Scott E. Youlten & John P. Kemp & John G. Logan & Elena J. Ghirardello & Claudio M. Sergio & Michael R. G. Dack & Siobhan E. Guilfoyle & Victoria D. Leitch & Natalie C. Butterfield & Davide Komla-Ebri, 2021. "Osteocyte transcriptome mapping identifies a molecular landscape controlling skeletal homeostasis and susceptibility to skeletal disease," Nature Communications, Nature, vol. 12(1), pages 1-21, December.
    2. Young-Yun Kong & Hiroki Yoshida & Ildiko Sarosi & Hong-Lin Tan & Emma Timms & Casey Capparelli & Sean Morony & Antonio J. Oliveira-dos-Santos & Gwyneth Van & Annick Itie & Wilson Khoo & Andrew Wakeham, 1999. "OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis," Nature, Nature, vol. 397(6717), pages 315-323, January.
    3. Maki Uenaka & Erika Yamashita & Junichi Kikuta & Akito Morimoto & Tomoka Ao & Hiroki Mizuno & Masayuki Furuya & Tetsuo Hasegawa & Hiroyuki Tsukazaki & Takao Sudo & Keizo Nishikawa & Daisuke Okuzaki & , 2022. "Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Masayuki Furuya & Junichi Kikuta & Sayumi Fujimori & Shigeto Seno & Hiroki Maeda & Mai Shirazaki & Maki Uenaka & Hiroki Mizuno & Yoriko Iwamoto & Akito Morimoto & Kunihiko Hashimoto & Takeshi Ito & Yu, 2018. "Direct cell–cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    5. Christian E. Jacome-Galarza & Gulce I. Percin & James T. Muller & Elvira Mass & Tomi Lazarov & Jiri Eitler & Martina Rauner & Vijay K. Yadav & Lucile Crozet & Mathieu Bohm & Pierre-Louis Loyher & Gera, 2019. "Developmental origin, functional maintenance and genetic rescue of osteoclasts," Nature, Nature, vol. 568(7753), pages 541-545, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Jialiang S. Wang & Tushar Kamath & Courtney M. Mazur & Fatemeh Mirzamohammadi & Daniel Rotter & Hironori Hojo & Christian D. Castro & Nicha Tokavanich & Rushi Patel & Nicolas Govea & Tetsuya Enishi & , 2021. "Control of osteocyte dendrite formation by Sp7 and its target gene osteocrin," Nature Communications, Nature, vol. 12(1), pages 1-20, December.
    2. Tang-Chuan Wang & Che-Chen Lin & Chia-Der Lin & Hsiung-Kwang Chung & Ching-Yuang Wang & Ming-Hsui Tsai & Chia-Hung Kao, 2015. "Increased Acquired Cholesteatoma Risk in Patients with Osteoporosis: A Retrospective Cohort Study," PLOS ONE, Public Library of Science, vol. 10(7), pages 1-10, July.
    3. Maki Uenaka & Erika Yamashita & Junichi Kikuta & Akito Morimoto & Tomoka Ao & Hiroki Mizuno & Masayuki Furuya & Tetsuo Hasegawa & Hiroyuki Tsukazaki & Takao Sudo & Keizo Nishikawa & Daisuke Okuzaki & , 2022. "Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Darja Andreev & Katerina Kachler & Mengdan Liu & Zhu Chen & Brenda Krishnacoumar & Mark Ringer & Silke Frey & Gerhard Krönke & David Voehringer & Georg Schett & Aline Bozec, 2024. "Eosinophils preserve bone homeostasis by inhibiting excessive osteoclast formation and activity via eosinophil peroxidase," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    5. Peng Ding & Chuan Gao & Jian Zhou & Jialun Mei & Gan Li & Delin Liu & Hao Li & Peng Liao & Meng Yao & Bingqi Wang & Yafei Lu & Xiaoyuan Peng & Chenyi Jiang & Jimin Yin & Yigang Huang & Minghao Zheng &, 2024. "Mitochondria from osteolineage cells regulate myeloid cell-mediated bone resorption," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    6. M. Gabriele Bixel & Kishor K. Sivaraj & Melanie Timmen & Vishal Mohanakrishnan & Anusha Aravamudhan & Susanne Adams & Bong-Ihn Koh & Hyun-Woo Jeong & Kai Kruse & Richard Stange & Ralf H. Adams, 2024. "Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration," Nature Communications, Nature, vol. 15(1), pages 1-22, December.
    7. Yang Zhao & Jingyuan Ning & Hongqi Teng & Yalan Deng & Marisela Sheldon & Lei Shi & Consuelo Martinez & Jie Zhang & Annie Tian & Yutong Sun & Shinichi Nakagawa & Fan Yao & Hai Wang & Li Ma, 2024. "Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    8. Jarred M. Whitlock & Evgenia Leikina & Kamran Melikov & Luis Fernandez Castro & Sandy Mattijssen & Richard J. Maraia & Michael T. Collins & Leonid V. Chernomordik, 2023. "Cell surface-bound La protein regulates the cell fusion stage of osteoclastogenesis," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-44000-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.