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Direct cell–cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo

Author

Listed:
  • Masayuki Furuya

    (Osaka University
    Osaka University
    Osaka University)

  • Junichi Kikuta

    (Osaka University
    Osaka University)

  • Sayumi Fujimori

    (Osaka University)

  • Shigeto Seno

    (Osaka University)

  • Hiroki Maeda

    (Osaka University)

  • Mai Shirazaki

    (Osaka University
    Osaka University)

  • Maki Uenaka

    (Osaka University
    Osaka University)

  • Hiroki Mizuno

    (Osaka University
    Osaka University)

  • Yoriko Iwamoto

    (Osaka University
    Osaka University)

  • Akito Morimoto

    (Osaka University
    Osaka University)

  • Kunihiko Hashimoto

    (Osaka University
    Osaka University
    Osaka University)

  • Takeshi Ito

    (Asahi Kasei Pharma Corporation)

  • Yukihiro Isogai

    (Asahi Kasei Pharma Corporation)

  • Masafumi Kashii

    (Osaka University)

  • Takashi Kaito

    (Osaka University)

  • Shinsuke Ohba

    (The University of Tokyo)

  • Ung-il Chung

    (The University of Tokyo)

  • Alexander C. Lichtler

    (University of Connecticut Health Center)

  • Kazuya Kikuchi

    (Osaka University)

  • Hideo Matsuda

    (Osaka University)

  • Hideki Yoshikawa

    (Osaka University)

  • Masaru Ishii

    (Osaka University
    Osaka University)

Abstract

Bone homeostasis is regulated by communication between bone-forming mature osteoblasts (mOBs) and bone-resorptive mature osteoclasts (mOCs). However, the spatial–temporal relationship and mode of interaction in vivo remain elusive. Here we show, by using an intravital imaging technique, that mOB and mOC functions are regulated via direct cell–cell contact between these cell types. The mOBs and mOCs mainly occupy discrete territories in the steady state, although direct cell–cell contact is detected in spatiotemporally limited areas. In addition, a pH-sensing fluorescence probe reveals that mOCs secrete protons for bone resorption when they are not in contact with mOBs, whereas mOCs contacting mOBs are non-resorptive, suggesting that mOBs can inhibit bone resorption by direct contact. Intermittent administration of parathyroid hormone causes bone anabolic effects, which lead to a mixed distribution of mOBs and mOCs, and increase cell–cell contact. This study reveals spatiotemporal intercellular interactions between mOBs and mOCs affecting bone homeostasis in vivo.

Suggested Citation

  • Masayuki Furuya & Junichi Kikuta & Sayumi Fujimori & Shigeto Seno & Hiroki Maeda & Mai Shirazaki & Maki Uenaka & Hiroki Mizuno & Yoriko Iwamoto & Akito Morimoto & Kunihiko Hashimoto & Takeshi Ito & Yu, 2018. "Direct cell–cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-017-02541-w
    DOI: 10.1038/s41467-017-02541-w
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    Cited by:

    1. Maki Uenaka & Erika Yamashita & Junichi Kikuta & Akito Morimoto & Tomoka Ao & Hiroki Mizuno & Masayuki Furuya & Tetsuo Hasegawa & Hiroyuki Tsukazaki & Takao Sudo & Keizo Nishikawa & Daisuke Okuzaki & , 2022. "Osteoblast-derived vesicles induce a switch from bone-formation to bone-resorption in vivo," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. M. Gabriele Bixel & Kishor K. Sivaraj & Melanie Timmen & Vishal Mohanakrishnan & Anusha Aravamudhan & Susanne Adams & Bong-Ihn Koh & Hyun-Woo Jeong & Kai Kruse & Richard Stange & Ralf H. Adams, 2024. "Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration," Nature Communications, Nature, vol. 15(1), pages 1-22, December.
    3. Peng Ding & Chuan Gao & Jian Zhou & Jialun Mei & Gan Li & Delin Liu & Hao Li & Peng Liao & Meng Yao & Bingqi Wang & Yafei Lu & Xiaoyuan Peng & Chenyi Jiang & Jimin Yin & Yigang Huang & Minghao Zheng &, 2024. "Mitochondria from osteolineage cells regulate myeloid cell-mediated bone resorption," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    4. Yongkuk Park & Tadatoshi Sato & Jungwoo Lee, 2023. "Functional and analytical recapitulation of osteoclast biology on demineralized bone paper," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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