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Regulation of the cell division hydrolase RipC by the FtsEX system in Mycobacterium tuberculosis

Author

Listed:
  • Jianwei Li

    (National University of Singapore)

  • Xin Xu

    (National University of Singapore)

  • Jian Shi

    (National University of Singapore)

  • Juan A. Hermoso

    (Consejo Superior de Investigaciones Científicas)

  • Lok-To Sham

    (National University of Singapore)

  • Min Luo

    (National University of Singapore
    National University of Singapore)

Abstract

The FtsEX complex regulates, directly or via a protein mediator depending on bacterial genera, peptidoglycan degradation for cell division. In mycobacteria and Gram-positive bacteria, the FtsEX system directly activates peptidoglycan-hydrolases by a mechanism that remains unclear. Here we report our investigation of Mycobacterium tuberculosis FtsEX as a non-canonical regulator with high basal ATPase activity. The cryo-EM structures of the FtsEX system alone and in complex with RipC, as well as the ATP-activated state, unveil detailed information on the signal transduction mechanism, leading to the activation of RipC. Our findings indicate that RipC is recognized through a “Match and Fit” mechanism, resulting in an asymmetric rearrangement of the extracellular domains of FtsX and a unique inclined binding mode of RipC. This study provides insights into the molecular mechanisms of FtsEX and RipC regulation in the context of a critical human pathogen, guiding the design of drugs targeting peptidoglycan remodeling.

Suggested Citation

  • Jianwei Li & Xin Xu & Jian Shi & Juan A. Hermoso & Lok-To Sham & Min Luo, 2023. "Regulation of the cell division hydrolase RipC by the FtsEX system in Mycobacterium tuberculosis," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43770-6
    DOI: 10.1038/s41467-023-43770-6
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