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Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation

Author

Listed:
  • Rebecca J. Harris

    (Newcastle University)

  • Maninder Heer

    (Newcastle University)

  • Mark D. Levasseur

    (Newcastle University)

  • Tyrell N. Cartwright

    (Newcastle University)

  • Bethany Weston

    (Newcastle University)

  • Jennifer L. Mitchell

    (Newcastle University)

  • Jonathan M. Coxhead

    (Newcastle University)

  • Luke Gaughan

    (Newcastle University
    Newcastle University Centre for Cancer, Faculty of Medical Sciences)

  • Lisa Prendergast

    (Newcastle University)

  • Daniel Rico

    (Newcastle University
    Newcastle University Centre for Cancer, Faculty of Medical Sciences
    CSIC-Universidad Sevilla-Universidad Pablo de Olavide-Junta de Andalucía)

  • Jonathan M. G. Higgins

    (Newcastle University
    Newcastle University Centre for Cancer, Faculty of Medical Sciences)

Abstract

Histone modifications influence the recruitment of reader proteins to chromosomes to regulate events including transcription and cell division. The idea of a histone code, where combinations of modifications specify unique downstream functions, is widely accepted and can be demonstrated in vitro. For example, on synthetic peptides, phosphorylation of Histone H3 at threonine-3 (H3T3ph) prevents the binding of reader proteins that recognize trimethylation of the adjacent lysine-4 (H3K4me3), including the TAF3 component of TFIID. To study these combinatorial effects in cells, we analyzed the genome-wide distribution of H3T3ph and H3K4me2/3 during mitosis. We find that H3T3ph anti-correlates with adjacent H3K4me2/3 in cells, and that the PHD domain of TAF3 can bind H3K4me2/3 in isolated mitotic chromatin despite the presence of H3T3ph. Unlike in vitro, H3K4 readers are still displaced from chromosomes in mitosis in Haspin-depleted cells lacking H3T3ph. H3T3ph is therefore unlikely to be responsible for transcriptional downregulation during cell division.

Suggested Citation

  • Rebecca J. Harris & Maninder Heer & Mark D. Levasseur & Tyrell N. Cartwright & Bethany Weston & Jennifer L. Mitchell & Jonathan M. Coxhead & Luke Gaughan & Lisa Prendergast & Daniel Rico & Jonathan M., 2023. "Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43115-3
    DOI: 10.1038/s41467-023-43115-3
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    References listed on IDEAS

    as
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