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Regulation of HP1–chromatin binding by histone H3 methylation and phosphorylation

Author

Listed:
  • Wolfgang Fischle

    (Laboratory of Chromatin Biology)

  • Boo Shan Tseng

    (The Rockefeller University)

  • Holger L. Dormann

    (Laboratory of Chromatin Biology)

  • Beatrix M. Ueberheide

    (Departments of Chemistry)

  • Benjamin A. Garcia

    (Departments of Chemistry)

  • Jeffrey Shabanowitz

    (Departments of Chemistry)

  • Donald F. Hunt

    (Departments of Chemistry
    Pathology, University of Virginia)

  • Hironori Funabiki

    (The Rockefeller University)

  • C. David Allis

    (Laboratory of Chromatin Biology)

Abstract

Tri-methylation of histone H3 lysine 9 is important for recruiting heterochromatin protein 1 (HP1) to discrete regions of the genome, thereby regulating gene expression, chromatin packaging and heterochromatin formation. Here we show that HP1α, -β, and -γ are released from chromatin during the M phase of the cell cycle, even though tri-methylation levels of histone H3 lysine 9 remain unchanged. However, the additional, transient modification of histone H3 by phosphorylation of serine 10 next to the more stable methyl-lysine 9 mark is sufficient to eject HP1 proteins from their binding sites. Inhibition or depletion of the mitotic kinase Aurora B, which phosphorylates serine 10 on histone H3, causes retention of HP1 proteins on mitotic chromosomes, suggesting that H3 serine 10 phosphorylation is necessary for the dissociation of HP1 from chromatin in M phase. These findings establish a regulatory mechanism of protein–protein interactions, through a combinatorial readout of two adjacent post-translational modifications: a stable methylation and a dynamic phosphorylation mark.

Suggested Citation

  • Wolfgang Fischle & Boo Shan Tseng & Holger L. Dormann & Beatrix M. Ueberheide & Benjamin A. Garcia & Jeffrey Shabanowitz & Donald F. Hunt & Hironori Funabiki & C. David Allis, 2005. "Regulation of HP1–chromatin binding by histone H3 methylation and phosphorylation," Nature, Nature, vol. 438(7071), pages 1116-1122, December.
    • Handle: RePEc:nat:nature:v:438:y:2005:i:7071:d:10.1038_nature04219
    DOI: 10.1038/nature04219
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    Cited by:

    1. Rebecca J. Harris & Maninder Heer & Mark D. Levasseur & Tyrell N. Cartwright & Bethany Weston & Jennifer L. Mitchell & Jonathan M. Coxhead & Luke Gaughan & Lisa Prendergast & Daniel Rico & Jonathan M., 2023. "Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Judith H. I. Haarhuis & Robin H. Weide & Vincent A. Blomen & Koen D. Flach & Hans Teunissen & Laureen Willems & Thijn R. Brummelkamp & Benjamin D. Rowland & Elzo Wit, 2022. "A Mediator-cohesin axis controls heterochromatin domain formation," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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