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Capturing nascent extracellular vesicles by metabolic glycan labeling-assisted microfluidics

Author

Listed:
  • Qiuyue Wu

    (Xiamen University)

  • Wencheng Wang

    (Xiamen University)

  • Chi Zhang

    (Xiamen University)

  • Zhenlong You

    (Xiamen University)

  • Yinyan Zeng

    (Xiamen University)

  • Yinzhu Lu

    (Xiamen University)

  • Suhui Zhang

    (Xiamen University)

  • Xingrui Li

    (Xiamen University)

  • Chaoyong Yang

    (Xiamen University
    Shanghai Jiao Tong University School of Medicine)

  • Yanling Song

    (Xiamen University)

Abstract

Extracellular vesicle (EV) secretion is a dynamic process crucial to cellular communication. Temporally sorting EVs, i.e., separating the newly-produced ones from the pre-existing, can allow not only deep understanding of EV dynamics, but also the discovery of potential EV biomarkers that are related to disease progression or responsible to drug intervention. However, the high similarity between the nascent and pre-existing EVs makes temporal separation extremely challenging. Here, by co-translational introduction of azido groups to act as a timestamp for click chemistry labelling, we develop a microfluidic-based strategy to enable selective isolation of nascent EVs stimulated by an external cue. In two mouse models of anti-PD-L1 immunotherapy, we demonstrate the strategy’s feasibility and reveal the high positive correlation of nascent PD-L1+ EV level to tumor volume, suggesting an important role of nascent EVs in response to immunotherapy in cancer treatment.

Suggested Citation

  • Qiuyue Wu & Wencheng Wang & Chi Zhang & Zhenlong You & Yinyan Zeng & Yinzhu Lu & Suhui Zhang & Xingrui Li & Chaoyong Yang & Yanling Song, 2023. "Capturing nascent extracellular vesicles by metabolic glycan labeling-assisted microfluidics," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42248-9
    DOI: 10.1038/s41467-023-42248-9
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    References listed on IDEAS

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