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Click-electrochemistry for the rapid labeling of virus, bacteria and cell surfaces

Author

Listed:
  • Sébastien Depienne

    (Nantes Université, CNRS, CEISAM UMR 6230)

  • Mohammed Bouzelha

    (Nantes Université, TaRGeT, Translational Research for Gene Therapies, CHU Nantes, INSERM, UMR 1089)

  • Emmanuelle Courtois

    (Nantes Université, CNRS, US2B, UMR 6286)

  • Karine Pavageau

    (Nantes Université, TaRGeT, Translational Research for Gene Therapies, CHU Nantes, INSERM, UMR 1089)

  • Pierre-Alban Lalys

    (Nantes Université, CNRS, CEISAM UMR 6230)

  • Maia Marchand

    (Nantes Université, CNRS, CEISAM UMR 6230)

  • Dimitri Alvarez-Dorta

    (Nantes Université, CNRS, CEISAM UMR 6230
    Capacités, 16 rue des marchandises)

  • Steven Nedellec

    (Nantes Université, CHU Nantes, CNRS, Inserm, BioCore, US16, SFR Bonamy)

  • Laura Marín-Fernández

    (Nantes Université, CNRS, US2B, UMR 6286)

  • Cyrille Grandjean

    (Nantes Université, CNRS, US2B, UMR 6286)

  • Mohammed Boujtita

    (Nantes Université, CNRS, CEISAM UMR 6230)

  • David Deniaud

    (Nantes Université, CNRS, CEISAM UMR 6230)

  • Mathieu Mével

    (Nantes Université, TaRGeT, Translational Research for Gene Therapies, CHU Nantes, INSERM, UMR 1089)

  • Sébastien G. Gouin

    (Nantes Université, CNRS, CEISAM UMR 6230)

Abstract

Methods for direct covalent ligation of microorganism surfaces remain poorly reported, and mostly based on metabolic engineering for bacteria and cells functionalization. While effective, a faster method avoiding the bio-incorporation step would be highly complementary. Here, we used N-methylluminol (NML), a fully tyrosine-selective protein anchoring group after one-electron oxidation, to label the surface of viruses, living bacteria and cells. The functionalization was performed electrochemically and in situ by applying an electric potential to aqueous buffered solutions of tagged NML containing the viruses, bacteria or cells. The broad applicability of the click-electrochemistry method was explored on recombinant adeno-associated viruses (rAAV2), Escherichia coli (Gram-) and Staphyloccocus epidermidis (Gram + ) bacterial strains, and HEK293 and HeLa eukaryotic cell lines. Surface electro-conjugation was achieved in minutes to yield functionalized rAAV2 that conserved both structural integrity and infectivity properties, and living bacteria and cell lines that were still alive and able to divide.

Suggested Citation

  • Sébastien Depienne & Mohammed Bouzelha & Emmanuelle Courtois & Karine Pavageau & Pierre-Alban Lalys & Maia Marchand & Dimitri Alvarez-Dorta & Steven Nedellec & Laura Marín-Fernández & Cyrille Grandjea, 2023. "Click-electrochemistry for the rapid labeling of virus, bacteria and cell surfaces," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40534-0
    DOI: 10.1038/s41467-023-40534-0
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    References listed on IDEAS

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    1. Jennifer A. Prescher & Danielle H. Dube & Carolyn R. Bertozzi, 2004. "Chemical remodelling of cell surfaces in living animals," Nature, Nature, vol. 430(7002), pages 873-877, August.
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