IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-41285-8.html
   My bibliography  Save this article

A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles

Author

Listed:
  • Kangfu Chen

    (University of Toronto)

  • Bill T. V. Duong

    (University of Toronto)

  • Sharif U. Ahmed

    (University of Toronto)

  • Piriththiv Dhavarasa

    (University of Toronto)

  • Zongjie Wang

    (Northwestern University)

  • Mahmoud Labib

    (University of Toronto
    Northwestern University
    University of Plymouth)

  • Connor Flynn

    (University of Toronto
    Northwestern University)

  • Jingya Xu

    (University of Toronto)

  • Yi Y. Zhang

    (University of Toronto)

  • Hansen Wang

    (University of Toronto)

  • Xiaolong Yang

    (University of Toronto)

  • Jagotamoy Das

    (Northwestern University)

  • Hossein Zargartalebi

    (University of Toronto)

  • Yuan Ma

    (University of Toronto)

  • Shana O. Kelley

    (University of Toronto
    University of Toronto
    University of Toronto
    Northwestern University)

Abstract

Exosomal PD-L1 (exoPD-L1) has recently received significant attention as a biomarker predicting immunotherapeutic responses involving the PD1/PD-L1 pathway. However, current technologies for exosomal analysis rely primarily on bulk measurements that do not consider the heterogeneity found within exosomal subpopulations. Here, we present a nanoscale cytometry platform NanoEPIC, enabling phenotypic sorting and exoPD-L1 profiling from blood plasma. We highlight the efficacy of NanoEPIC in monitoring anti-PD-1 immunotherapy through the interrogation of exoPD-L1. NanoEPIC generates signature exoPD-L1 patterns in responders and non-responders. In mice treated with PD1-targeted immunotherapy, exoPD-L1 is correlated with tumor growth, PD-L1 burden in tumors, and the immune suppression of CD8+ tumor-infiltrating lymphocytes. Small extracellular vesicles (sEVs) with different PD-L1 expression levels display distinctive inhibitory effects on CD8 + T cells. NanoEPIC offers robust, high-throughput profiling of exosomal markers, enabling sEV subpopulation analysis. This platform holds the potential for enhanced cancer screening, personalized treatment, and therapeutic response monitoring.

Suggested Citation

  • Kangfu Chen & Bill T. V. Duong & Sharif U. Ahmed & Piriththiv Dhavarasa & Zongjie Wang & Mahmoud Labib & Connor Flynn & Jingya Xu & Yi Y. Zhang & Hansen Wang & Xiaolong Yang & Jagotamoy Das & Hossein , 2023. "A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41285-8
    DOI: 10.1038/s41467-023-41285-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-41285-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-41285-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Huilin Shao & Jaehoon Chung & Kyungheon Lee & Leonora Balaj & Changwook Min & Bob S. Carter & Fred H. Hochberg & Xandra O. Breakefield & Hakho Lee & Ralph Weissleder, 2015. "Chip-based analysis of exosomal mRNA mediating drug resistance in glioblastoma," Nature Communications, Nature, vol. 6(1), pages 1-9, November.
    2. Gang Chen & Alexander C. Huang & Wei Zhang & Gao Zhang & Min Wu & Wei Xu & Zili Yu & Jiegang Yang & Beike Wang & Honghong Sun & Houfu Xia & Qiwen Man & Wenqun Zhong & Leonardo F. Antelo & Bin Wu & Xue, 2018. "Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response," Nature, Nature, vol. 560(7718), pages 382-386, August.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Qiuyue Wu & Wencheng Wang & Chi Zhang & Zhenlong You & Yinyan Zeng & Yinzhu Lu & Suhui Zhang & Xingrui Li & Chaoyong Yang & Yanling Song, 2023. "Capturing nascent extracellular vesicles by metabolic glycan labeling-assisted microfluidics," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Lei Guan & Bin Wu & Ting Li & Lynn A. Beer & Gaurav Sharma & Mingyue Li & Chin Nien Lee & Shujing Liu & Changsong Yang & Lili Huang & Dennie T. Frederick & Genevieve M. Boland & Guangcan Shao & Tatyan, 2022. "HRS phosphorylation drives immunosuppressive exosome secretion and restricts CD8+ T-cell infiltration into tumors," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Di-Ao Liu & Kai Tao & Bin Wu & Ziyan Yu & Malwina Szczepaniak & Matthew Rames & Changsong Yang & Tatyana Svitkina & Yueyao Zhu & Fengyuan Xu & Xiaolin Nan & Wei Guo, 2023. "A phosphoinositide switch mediates exocyst recruitment to multivesicular endosomes for exosome secretion," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Feng Xie & Xiaoxue Zhou & Peng Su & Heyu Li & Yifei Tu & Jinjin Du & Chen Pan & Xiang Wei & Min Zheng & Ke Jin & Liyan Miao & Chao Wang & Xuli Meng & Hans Dam & Peter Dijke & Long Zhang & Fangfang Zho, 2022. "Breast cancer cell-derived extracellular vesicles promote CD8+ T cell exhaustion via TGF-β type II receptor signaling," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    5. Lin-Zhou Zhang & Jie-Gang Yang & Gai-Li Chen & Qi-Hui Xie & Qiu-Yun Fu & Hou-Fu Xia & Yi-Cun Li & Jue Huang & Ye Li & Min Wu & Hai-Ming Liu & Fu-Bing Wang & Ke-Zhen Yi & Huan-Gang Jiang & Fu-Xiang Zho, 2024. "PD-1/CD80+ small extracellular vesicles from immunocytes induce cold tumours featured with enhanced adaptive immunosuppression," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    6. Yan Zhang & Chi Yan Wong & Carine Z. J. Lim & Qingchang Chen & Zhonglang Yu & Auginia Natalia & Zhigang Wang & Qing You Pang & See Wee Lim & Tze Ping Loh & Beng Ti Ang & Carol Tang & Huilin Shao, 2023. "Multiplexed RNA profiling by regenerative catalysis enables blood-based subtyping of brain tumors," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41285-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.