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Control of intracellular pH and bicarbonate by CO2 diffusion into human sperm

Author

Listed:
  • Elena Grahn

    (Molecular Sensory Systems)

  • Svenja V. Kaufmann

    (Bioanalytical Mass Spectrometry)

  • Malika Askarova

    (Molecular Sensory Systems)

  • Momchil Ninov

    (Bioanalytical Mass Spectrometry
    Institute of Clinical Chemistry, Bioanalytics)

  • Luisa M. Welp

    (Bioanalytical Mass Spectrometry
    Institute of Clinical Chemistry, Bioanalytics)

  • Thomas K. Berger

    (Molecular Sensory Systems
    Philipps-University Marburg)

  • Henning Urlaub

    (Bioanalytical Mass Spectrometry
    Institute of Clinical Chemistry, Bioanalytics
    Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells (MBExC), University of Göttingen)

  • U.Benjamin Kaupp

    (Molecular Sensory Systems
    University Bonn)

Abstract

The reaction of CO2 with H2O to form bicarbonate (HCO3−) and H+ controls sperm motility and fertilization via HCO3−-stimulated cAMP synthesis. A complex network of signaling proteins participates in this reaction. Here, we identify key players that regulate intracellular pH (pHi) and HCO3− in human sperm by quantitative mass spectrometry (MS) and kinetic patch-clamp fluorometry. The resting pHi is set by amiloride-sensitive Na+/H+ exchange. The sperm-specific putative Na+/H+ exchanger SLC9C1, unlike its sea urchin homologue, is not gated by voltage or cAMP. Transporters and channels implied in HCO3− transport are not detected, and may be present at copy numbers

Suggested Citation

  • Elena Grahn & Svenja V. Kaufmann & Malika Askarova & Momchil Ninov & Luisa M. Welp & Thomas K. Berger & Henning Urlaub & U.Benjamin Kaupp, 2023. "Control of intracellular pH and bicarbonate by CO2 diffusion into human sperm," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40855-0
    DOI: 10.1038/s41467-023-40855-0
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    References listed on IDEAS

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