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NvPrdm14d-expressing neural progenitor cells contribute to non-ectodermal neurogenesis in Nematostella vectensis

Author

Listed:
  • Quentin I. B. Lemaître

    (University of Bergen)

  • Natascha Bartsch

    (University of Bergen
    University of Bergen)

  • Ian U. Kouzel

    (University of Bergen)

  • Henriette Busengdal

    (University of Bergen)

  • Gemma Sian Richards

    (University of Bergen)

  • Patrick R. H. Steinmetz

    (University of Bergen)

  • Fabian Rentzsch

    (University of Bergen
    University of Bergen)

Abstract

Neurogenesis has been studied extensively in the ectoderm, from which most animals generate the majority of their neurons. Neurogenesis from non-ectodermal tissue is, in contrast, poorly understood. Here we use the cnidarian Nematostella vectensis as a model to provide new insights into the molecular regulation of non-ectodermal neurogenesis. We show that the transcription factor NvPrdm14d is expressed in a subpopulation of NvSoxB(2)-expressing endodermal progenitor cells and their NvPOU4-expressing progeny. Using a new transgenic reporter line, we show that NvPrdm14d-expressing cells give rise to neurons in the body wall and in close vicinity of the longitudinal retractor muscles. RNA-sequencing of NvPrdm14d::GFP-expressing cells and gene knockdown experiments provide candidate genes for the development and function of these neurons. Together, the identification of a population of endoderm-specific neural progenitor cells and of previously undescribed putative motoneurons in Nematostella provide new insights into the regulation of non-ectodermal neurogenesis.

Suggested Citation

  • Quentin I. B. Lemaître & Natascha Bartsch & Ian U. Kouzel & Henriette Busengdal & Gemma Sian Richards & Patrick R. H. Steinmetz & Fabian Rentzsch, 2023. "NvPrdm14d-expressing neural progenitor cells contribute to non-ectodermal neurogenesis in Nematostella vectensis," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39789-4
    DOI: 10.1038/s41467-023-39789-4
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    References listed on IDEAS

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    2. James M. Gahan & Ian U. Kouzel & Kamilla Ormevik Jansen & Pawel Burkhardt & Fabian Rentzsch, 2022. "Histone demethylase Lsd1 is required for the differentiation of neural cells in Nematostella vectensis," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
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