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A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity

Author

Listed:
  • Na-Yu Chia

    (Gene Regulation Laboratory, Genome Institute of Singapore
    School of Biological Sciences, Nanyang Technological University)

  • Yun-Shen Chan

    (Gene Regulation Laboratory, Genome Institute of Singapore
    Graduate School for Integrative Sciences & Engineering, National University of Singapore)

  • Bo Feng

    (Gene Regulation Laboratory, Genome Institute of Singapore)

  • Xinyi Lu

    (Gene Regulation Laboratory, Genome Institute of Singapore
    Dept of Biological Sciences, National University of Singapore)

  • Yuriy L. Orlov

    (Computational and Systems Biology group, Genome Institute of Singapore)

  • Dimitri Moreau

    (Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673)

  • Pankaj Kumar

    (Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673)

  • Lin Yang

    (Gene Regulation Laboratory, Genome Institute of Singapore)

  • Jianming Jiang

    (Gene Regulation Laboratory, Genome Institute of Singapore)

  • Mei-Sheng Lau

    (Gene Regulation Laboratory, Genome Institute of Singapore)

  • Mikael Huss

    (Computational and Systems Biology group, Genome Institute of Singapore)

  • Boon-Seng Soh

    (Stem Cell and Developmental Biology, Genome Institute of Singapore)

  • Petra Kraus

    (Stem Cell and Developmental Biology, Genome Institute of Singapore)

  • Pin Li

    (Stem Cell and Developmental Biology, Genome Institute of Singapore)

  • Thomas Lufkin

    (Stem Cell and Developmental Biology, Genome Institute of Singapore)

  • Bing Lim

    (Stem Cell and Developmental Biology, Genome Institute of Singapore
    Center for Life Sciences, Harvard Medical School, 330 Brookline Avenue, Boston, Massachusetts 02115, USA)

  • Neil D. Clarke

    (Computational and Systems Biology group, Genome Institute of Singapore
    National University of Singapore, 8 Medical Drive, Yong Loo Lin School of Medicine)

  • Frederic Bard

    (Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673
    National University of Singapore, 8 Medical Drive, Yong Loo Lin School of Medicine)

  • Huck-Hui Ng

    (Gene Regulation Laboratory, Genome Institute of Singapore
    School of Biological Sciences, Nanyang Technological University
    Graduate School for Integrative Sciences & Engineering, National University of Singapore
    Dept of Biological Sciences, National University of Singapore)

Abstract

Screen test for stem-cell cues If the full potential of human embryonic stem (ES) cells in research and clinical applications is to be realized, we need a detailed understanding of the genetic network that governs their unique properties. A genome-wide RNA interference screen identifies a wealth of regulators of self-renewal and pluripotency properties in human embryonic stem cells. PRDM14, for example, represents a key transcription factor required for the maintenance of human ES cell identity and reprogramming of somatic cells to pluripotency.

Suggested Citation

  • Na-Yu Chia & Yun-Shen Chan & Bo Feng & Xinyi Lu & Yuriy L. Orlov & Dimitri Moreau & Pankaj Kumar & Lin Yang & Jianming Jiang & Mei-Sheng Lau & Mikael Huss & Boon-Seng Soh & Petra Kraus & Pin Li & Thom, 2010. "A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity," Nature, Nature, vol. 468(7321), pages 316-320, November.
    • Handle: RePEc:nat:nature:v:468:y:2010:i:7321:d:10.1038_nature09531
    DOI: 10.1038/nature09531
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    Cited by:

    1. Bess P. Rosen & Qing V. Li & Hyein S. Cho & Dingyu Liu & Dapeng Yang & Sarah Graff & Jielin Yan & Renhe Luo & Nipun Verma & Jeyaram R. Damodaran & Hanuman T. Kale & Samuel J. Kaplan & Michael A. Beer , 2024. "Parallel genome-scale CRISPR-Cas9 screens uncouple human pluripotent stem cell identity versus fitness," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    2. Quentin I. B. Lemaître & Natascha Bartsch & Ian U. Kouzel & Henriette Busengdal & Gemma Sian Richards & Patrick R. H. Steinmetz & Fabian Rentzsch, 2023. "NvPrdm14d-expressing neural progenitor cells contribute to non-ectodermal neurogenesis in Nematostella vectensis," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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