IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-38457-x.html
   My bibliography  Save this article

saRNA vaccine expressing membrane-anchored RBD elicits broad and durable immunity against SARS-CoV-2 variants of concern

Author

Listed:
  • Mai Komori

    (VLP Therapeutics)

  • Takuto Nogimori

    (National Institutes of Biomedical Innovation, Health, and Nutrition)

  • Amber L. Morey

    (VLP Therapeutics)

  • Takashi Sekida

    (VLP Therapeutics Japan)

  • Keiko Ishimoto

    (VLP Therapeutics)

  • Matthew R. Hassett

    (VLP Therapeutics)

  • Yuji Masuta

    (National Institutes of Biomedical Innovation, Health, and Nutrition)

  • Hirotaka Ode

    (National Hospital Organization Nagoya Medical Center)

  • Tomokazu Tamura

    (Hokkaido University)

  • Rigel Suzuki

    (Hokkaido University)

  • Jeff Alexander

    (VLP Therapeutics)

  • Yasutoshi Kido

    (Osaka Metropolitan University)

  • Kenta Matsuda

    (VLP Therapeutics)

  • Takasuke Fukuhara

    (Hokkaido University)

  • Yasumasa Iwatani

    (National Hospital Organization Nagoya Medical Center
    Nagoya University Graduate School of Medicine)

  • Takuya Yamamoto

    (National Institutes of Biomedical Innovation, Health, and Nutrition
    Osaka University
    Osaka University)

  • Jonathan F. Smith

    (VLP Therapeutics)

  • Wataru Akahata

    (VLP Therapeutics Japan)

Abstract

Several vaccines have been widely used to counteract the global pandemic caused by SARS-CoV-2. However, due to the rapid emergence of SARS-CoV-2 variants of concern (VOCs), further development of vaccines that confer broad and longer-lasting protection against emerging VOCs are needed. Here, we report the immunological characteristics of a self-amplifying RNA (saRNA) vaccine expressing the SARS-CoV-2 Spike (S) receptor binding domain (RBD), which is membrane-anchored by fusing with an N-terminal signal sequence and a C-terminal transmembrane domain (RBD-TM). Immunization with saRNA RBD-TM delivered in lipid nanoparticles (LNP) efficiently induces T-cell and B-cell responses in non-human primates (NHPs). In addition, immunized hamsters and NHPs are protected against SARS-CoV-2 challenge. Importantly, RBD-specific antibodies against VOCs are maintained for at least 12 months in NHPs. These findings suggest that this saRNA platform expressing RBD-TM will be a useful vaccine candidate inducing durable immunity against emerging SARS-CoV-2 strains.

Suggested Citation

  • Mai Komori & Takuto Nogimori & Amber L. Morey & Takashi Sekida & Keiko Ishimoto & Matthew R. Hassett & Yuji Masuta & Hirotaka Ode & Tomokazu Tamura & Rigel Suzuki & Jeff Alexander & Yasutoshi Kido & K, 2023. "saRNA vaccine expressing membrane-anchored RBD elicits broad and durable immunity against SARS-CoV-2 variants of concern," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38457-x
    DOI: 10.1038/s41467-023-38457-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-38457-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-38457-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Alexandra J. Spencer & Paul F. McKay & Sandra Belij-Rammerstorfer & Marta Ulaszewska & Cameron D. Bissett & Kai Hu & Karnyart Samnuan & Anna K. Blakney & Daniel Wright & Hannah R. Sharpe & Ciaran Gilb, 2021. "Heterologous vaccination regimens with self-amplifying RNA and adenoviral COVID vaccines induce robust immune responses in mice," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    2. Jun Lan & Jiwan Ge & Jinfang Yu & Sisi Shan & Huan Zhou & Shilong Fan & Qi Zhang & Xuanling Shi & Qisheng Wang & Linqi Zhang & Xinquan Wang, 2020. "Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor," Nature, Nature, vol. 581(7807), pages 215-220, May.
    3. Seiya Ozono & Yanzhao Zhang & Hirotaka Ode & Kaori Sano & Toong Seng Tan & Kazuo Imai & Kazuyasu Miyoshi & Satoshi Kishigami & Takamasa Ueno & Yasumasa Iwatani & Tadaki Suzuki & Kenzo Tokunaga, 2021. "SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    4. Paul F. McKay & Kai Hu & Anna K. Blakney & Karnyart Samnuan & Jonathan C. Brown & Rebecca Penn & Jie Zhou & Clément R. Bouton & Paul Rogers & Krunal Polra & Paulo J. C. Lin & Christopher Barbosa & Yin, 2020. "Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice," Nature Communications, Nature, vol. 11(1), pages 1-7, December.
    5. Amy R. Rappaport & Sue-Jean Hong & Ciaran D. Scallan & Leonid Gitlin & Arvin Akoopie & Gregory R. Boucher & Milana Egorova & J. Aaron Espinosa & Mario Fidanza & Melissa A. Kachura & Annie Shen & Glori, 2022. "Low-dose self-amplifying mRNA COVID-19 vaccine drives strong protective immunity in non-human primates against SARS-CoV-2 infection," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Nhân Thị Hồ & Steven G. Hughes & Van Thanh Ta & Lân Trọng Phan & Quyết Đỗ & Thượng Vũ Nguyễn & Anh Thị Văn Phạm & Mai Thị Ngọc Đặng & Lượng Viết Nguyễn & Quang Vinh Trịnh & Hùng Ngọc Phạm & Mến Văn Ch, 2024. "Safety, immunogenicity and efficacy of the self-amplifying mRNA ARCT-154 COVID-19 vaccine: pooled phase 1, 2, 3a and 3b randomized, controlled trials," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Tomokazu Tamura & Jumpei Ito & Keiya Uriu & Jiri Zahradnik & Izumi Kida & Yuki Anraku & Hesham Nasser & Maya Shofa & Yoshitaka Oda & Spyros Lytras & Naganori Nao & Yukari Itakura & Sayaka Deguchi & Ri, 2023. "Virological characteristics of the SARS-CoV-2 XBB variant derived from recombination of two Omicron subvariants," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    2. Pengcheng Han & Chao Su & Yanfang Zhang & Chongzhi Bai & Anqi Zheng & Chengpeng Qiao & Qing Wang & Sheng Niu & Qian Chen & Yuqin Zhang & Weiwei Li & Hanyi Liao & Jing Li & Zengyuan Zhang & Heecheol Ch, 2021. "Molecular insights into receptor binding of recent emerging SARS-CoV-2 variants," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    3. Tamara Kaleta & Lisa Kern & Samuel Leandro Hong & Martin Hölzer & Georg Kochs & Julius Beer & Daniel Schnepf & Martin Schwemmle & Nena Bollen & Philipp Kolb & Magdalena Huber & Svenja Ulferts & Sebast, 2022. "Antibody escape and global spread of SARS-CoV-2 lineage A.27," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Dennis Lapuente & Jana Fuchs & Jonas Willar & Ana Vieira Antão & Valentina Eberlein & Nadja Uhlig & Leila Issmail & Anna Schmidt & Friederike Oltmanns & Antonia Sophia Peter & Sandra Mueller-Schmucker, 2021. "Protective mucosal immunity against SARS-CoV-2 after heterologous systemic prime-mucosal boost immunization," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    5. Jun-Yu Si & Yuan-Mei Chen & Ye-Hui Sun & Meng-Xue Gu & Mei-Ling Huang & Lu-Lu Shi & Xiao Yu & Xiao Yang & Qing Xiong & Cheng-Bao Ma & Peng Liu & Zheng-Li Shi & Huan Yan, 2024. "Sarbecovirus RBD indels and specific residues dictating multi-species ACE2 adaptiveness," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    6. Hannah McClymont & Wenbiao Hu, 2021. "Weather Variability and COVID-19 Transmission: A Review of Recent Research," IJERPH, MDPI, vol. 18(2), pages 1-19, January.
    7. Ravindra B. Malabadi & Kiran P. Kolkar & Neelambika T. Meti & Raju K. Chalannavar, 2021. "Vaccine Development for Coronavirus (SARS-CoV-2) Disease (Covid-19); Lipid Nanoparticles," International Journal of Research and Scientific Innovation, International Journal of Research and Scientific Innovation (IJRSI), vol. 8(3), pages 189-195, March.
    8. Matthew R. Chang & Luke Tomasovic & Natalia A. Kuzmina & Adam J. Ronk & Patrick O. Byrne & Rebecca Johnson & Nadia Storm & Eduardo Olmedillas & Yixuan J. Hou & Alexandra Schäfer & Sarah R. Leist & Lon, 2022. "IgG-like bispecific antibodies with potent and synergistic neutralization against circulating SARS-CoV-2 variants of concern," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    9. Ioannis Kontoyiannis & Lambros Mertzanis & Athina Panotopoulou & Ioannis Papageorgiou & Maria Skoularidou, 2022. "Bayesian context trees: Modelling and exact inference for discrete time series," Journal of the Royal Statistical Society Series B, Royal Statistical Society, vol. 84(4), pages 1287-1323, September.
    10. Susan K. Vester & Rolle Rahikainen & Irsyad N. A. Khairil Anuar & Rory A. Hills & Tiong Kit Tan & Mark Howarth, 2022. "SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    11. Nikhil Kumar Tulsian & Raghuvamsi Venkata Palur & Xinlei Qian & Yue Gu & Bhuvaneshwari D/O Shunmuganathan & Firdaus Samsudin & Yee Hwa Wong & Jianqing Lin & Kiren Purushotorman & Mary McQueen Kozma & , 2023. "Defining neutralization and allostery by antibodies against COVID-19 variants," Nature Communications, Nature, vol. 14(1), pages 1-23, December.
    12. Neeltje van Doremalen & Jonathan E. Schulz & Danielle R. Adney & Taylor A. Saturday & Robert J. Fischer & Claude Kwe Yinda & Nazia Thakur & Joseph Newman & Marta Ulaszewska & Sandra Belij-Rammerstorfe, 2022. "ChAdOx1 nCoV-19 (AZD1222) or nCoV-19-Beta (AZD2816) protect Syrian hamsters against Beta Delta and Omicron variants," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    13. Jing Wang & Yuan-fei Pan & Li-fen Yang & Wei-hong Yang & Kexin Lv & Chu-ming Luo & Juan Wang & Guo-peng Kuang & Wei-chen Wu & Qin-yu Gou & Gen-yang Xin & Bo Li & Huan-le Luo & Shoudeng Chen & Yue-long, 2023. "Individual bat virome analysis reveals co-infection and spillover among bats and virus zoonotic potential," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    14. Jumpei Ito & Rigel Suzuki & Keiya Uriu & Yukari Itakura & Jiri Zahradnik & Kanako Terakado Kimura & Sayaka Deguchi & Lei Wang & Spyros Lytras & Tomokazu Tamura & Izumi Kida & Hesham Nasser & Maya Shof, 2023. "Convergent evolution of SARS-CoV-2 Omicron subvariants leading to the emergence of BQ.1.1 variant," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    15. Joseph Dodd-o & Abhishek Roy & Zain Siddiqui & Roya Jafari & Francesco Coppola & Santhamani Ramasamy & Afsal Kolloli & Dilip Kumar & Soni Kaundal & Boyang Zhao & Ranjeet Kumar & Alicia S. Robang & Jef, 2024. "Antiviral fibrils of self-assembled peptides with tunable compositions," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    16. Irena Cosic & Drasko Cosic & Ivan Loncarevic, 2021. "Analysis of UK and South African Strains of SARS-CoV-2 Using Resonant Recognition Model," International Journal of Sciences, Office ijSciences, vol. 10(03), pages 19-25, March.
    17. Cedric C. S. Tan & Jahcub Trew & Thomas P. Peacock & Kai Yi Mok & Charlie Hart & Kelvin Lau & Dongchun Ni & C. David L. Orme & Emma Ransome & William D. Pearse & Christopher M. Coleman & Dalan Bailey , 2023. "Genomic screening of 16 UK native bat species through conservationist networks uncovers coronaviruses with zoonotic potential," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    18. Hisano Yajima & Yuki Anraku & Yu Kaku & Kanako Terakado Kimura & Arnon Plianchaisuk & Kaho Okumura & Yoshiko Nakada-Nakura & Yusuke Atarashi & Takuya Hemmi & Daisuke Kuroda & Yoshimasa Takahashi & Shu, 2024. "Structural basis for receptor-binding domain mobility of the spike in SARS-CoV-2 BA.2.86 and JN.1," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    19. Kuan-Ying A. Huang & Xiaorui Chen & Arpita Mohapatra & Hong Thuy Vy Nguyen & Lisa Schimanski & Tiong Kit Tan & Pramila Rijal & Susan K. Vester & Rory A. Hills & Mark Howarth & Jennifer R. Keeffe & Ale, 2023. "Structural basis for a conserved neutralization epitope on the receptor-binding domain of SARS-CoV-2," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    20. Christopher C. Azubuike & Fay Couceiro & Samuel C. Robson & Maya Z. Piccinni & Joy E. M. Watts & John B. Williams & Anastasia J. Callaghan & Thomas P. Howard, 2022. "Developing Biosensors for SARS-CoV-2 Wastewater-Based Epidemiology: A Systematic Review of Trends, Limitations and Future Perspectives," Sustainability, MDPI, vol. 14(24), pages 1-23, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38457-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.