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Hydrogel-in-hydrogel live bioprinting for guidance and control of organoids and organotypic cultures

Author

Listed:
  • Anna Urciuolo

    (University of Padova
    Città della Speranza)

  • Giovanni Giuseppe Giobbe

    (University College London)

  • Yixiao Dong

    (ShanghaiTech University)

  • Federica Michielin

    (University College London)

  • Luca Brandolino

    (University of Padova
    Veneto Institute of Molecular Medicine)

  • Michael Magnussen

    (University College London)

  • Onelia Gagliano

    (University of Padova
    Veneto Institute of Molecular Medicine)

  • Giulia Selmin

    (University College London)

  • Valentina Scattolini

    (Città della Speranza)

  • Paolo Raffa

    (Città della Speranza)

  • Paola Caccin

    (University of Padova)

  • Soichi Shibuya

    (University College London)

  • Dominic Scaglioni

    (University College London)

  • Xuechun Wang

    (ShanghaiTech University)

  • Ju Qu

    (ShanghaiTech University)

  • Marko Nikolic

    (University College London)

  • Marco Montagner

    (University of Padova)

  • Gabriel L. Galea

    (University College London)

  • Hans Clevers

    (KNAW and University Medical Center
    Pharma Research and Early Development (pRED) of Roche)

  • Monica Giomo

    (University of Padova)

  • Paolo De Coppi

    (University College London
    Great Ormond Street Hospital)

  • Nicola Elvassore

    (University College London
    University of Padova
    Veneto Institute of Molecular Medicine)

Abstract

Three-dimensional hydrogel-based organ-like cultures can be applied to study development, regeneration, and disease in vitro. However, the control of engineered hydrogel composition, mechanical properties and geometrical constraints tends to be restricted to the initial time of fabrication. Modulation of hydrogel characteristics over time and according to culture evolution is often not possible. Here, we overcome these limitations by developing a hydrogel-in-hydrogel live bioprinting approach that enables the dynamic fabrication of instructive hydrogel elements within pre-existing hydrogel-based organ-like cultures. This can be achieved by crosslinking photosensitive hydrogels via two-photon absorption at any time during culture. We show that instructive hydrogels guide neural axon directionality in growing organotypic spinal cords, and that hydrogel geometry and mechanical properties control differential cell migration in developing cancer organoids. Finally, we show that hydrogel constraints promote cell polarity in liver organoids, guide small intestinal organoid morphogenesis and control lung tip bifurcation according to the hydrogel composition and shape.

Suggested Citation

  • Anna Urciuolo & Giovanni Giuseppe Giobbe & Yixiao Dong & Federica Michielin & Luca Brandolino & Michael Magnussen & Onelia Gagliano & Giulia Selmin & Valentina Scattolini & Paolo Raffa & Paola Caccin , 2023. "Hydrogel-in-hydrogel live bioprinting for guidance and control of organoids and organotypic cultures," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37953-4
    DOI: 10.1038/s41467-023-37953-4
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    References listed on IDEAS

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    1. Abdel Rahman Abdel Fattah & Niko Kolaitis & Katrien Daele & Brian Daza & Andika Gregorius Rustandi & Adrian Ranga, 2023. "Targeted mechanical stimulation via magnetic nanoparticles guides in vitro tissue development," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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