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Defining the early stages of intestinal colonisation by whipworms

Author

Listed:
  • María A. Duque-Correa

    (Wellcome Genome Campus
    University of Cambridge)

  • David Goulding

    (Wellcome Genome Campus)

  • Faye H. Rodgers

    (Wellcome Genome Campus
    Mogrify Ltd, 25 Cambridge Science Park)

  • J. Andrew Gillis

    (University of Cambridge)

  • Claire Cormie

    (Wellcome Genome Campus
    University of Cambridge)

  • Kate A. Rawlinson

    (Wellcome Genome Campus)

  • Allison J. Bancroft

    (Medicine and Health, University of Manchester)

  • Hayley M. Bennett

    (Wellcome Genome Campus
    Genentech, 1 DNA Way)

  • Magda E. Lotkowska

    (Wellcome Genome Campus)

  • Adam J. Reid

    (Wellcome Genome Campus
    University of Cambridge)

  • Anneliese O. Speak

    (Wellcome Genome Campus)

  • Paul Scott

    (Wellcome Genome Campus)

  • Nicholas Redshaw

    (Wellcome Genome Campus)

  • Charlotte Tolley

    (Wellcome Genome Campus)

  • Catherine McCarthy

    (Wellcome Genome Campus)

  • Cordelia Brandt

    (Wellcome Genome Campus)

  • Catherine Sharpe

    (Medicine and Health, University of Manchester
    InstilBio, UMIC Bio-Incubator)

  • Caroline Ridley

    (Medicine and Health, University of Manchester
    Prime Global Medical Communications)

  • Judit Gali Moya

    (University of Barcelona)

  • Claudia M. Carneiro

    (Federal University of Ouro Preto, Campus Universitario Morro do Cruzeiro)

  • Tobias Starborg

    (Medicine and Health, University of Manchester
    Rosalind Franklin Institute)

  • Kelly S. Hayes

    (Medicine and Health, University of Manchester)

  • Nancy Holroyd

    (Wellcome Genome Campus)

  • Mandy Sanders

    (Wellcome Genome Campus)

  • David J. Thornton

    (Medicine and Health, University of Manchester)

  • Richard K. Grencis

    (Medicine and Health, University of Manchester)

  • Matthew Berriman

    (Wellcome Genome Campus)

Abstract

Whipworms are large metazoan parasites that inhabit multi-intracellular epithelial tunnels in the large intestine of their hosts, causing chronic disease in humans and other mammals. How first-stage larvae invade host epithelia and establish infection remains unclear. Here we investigate early infection events using both Trichuris muris infections of mice and murine caecaloids, the first in-vitro system for whipworm infection and organoid model for live helminths. We show that larvae degrade mucus layers to access epithelial cells. In early syncytial tunnels, larvae are completely intracellular, woven through multiple live dividing cells. Using single-cell RNA sequencing of infected mouse caecum, we reveal that progression of infection results in cell damage and an expansion of enterocytes expressing of Isg15, potentially instigating the host immune response to the whipworm and tissue repair. Our results unravel intestinal epithelium invasion by whipworms and reveal specific host-parasite interactions that allow the whipworm to establish its multi-intracellular niche.

Suggested Citation

  • María A. Duque-Correa & David Goulding & Faye H. Rodgers & J. Andrew Gillis & Claire Cormie & Kate A. Rawlinson & Allison J. Bancroft & Hayley M. Bennett & Magda E. Lotkowska & Adam J. Reid & Annelies, 2022. "Defining the early stages of intestinal colonisation by whipworms," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29334-0
    DOI: 10.1038/s41467-022-29334-0
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