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Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

Author

Listed:
  • Giovanni Giuseppe Giobbe

    (University College London GOS Institute of Child Health)

  • Claire Crowley

    (University College London GOS Institute of Child Health)

  • Camilla Luni

    (ShanghaiTech University)

  • Sara Campinoti

    (University College London GOS Institute of Child Health
    the Francis Crick Institute)

  • Moustafa Khedr

    (University College London GOS Institute of Child Health)

  • Kai Kretzschmar

    (Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht)

  • Martina Maria De Santis

    (University College London GOS Institute of Child Health)

  • Elisa Zambaiti

    (University College London GOS Institute of Child Health)

  • Federica Michielin

    (University College London GOS Institute of Child Health)

  • Laween Meran

    (University College London GOS Institute of Child Health
    the Francis Crick Institute)

  • Qianjiang Hu

    (ShanghaiTech University)

  • Gijs van Son

    (Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht)

  • Luca Urbani

    (University College London GOS Institute of Child Health)

  • Anna Manfredi

    (Telethon Institute of Genetics and Medicine (TIGEM))

  • Monica Giomo

    (University of Padova)

  • Simon Eaton

    (University College London GOS Institute of Child Health)

  • Davide Cacchiarelli

    (Telethon Institute of Genetics and Medicine (TIGEM))

  • Vivian S. W. Li

    (the Francis Crick Institute)

  • Hans Clevers

    (Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht
    Princess Máxima Center (PMC) for Pediatric Oncology)

  • Paola Bonfanti

    (University College London GOS Institute of Child Health
    the Francis Crick Institute)

  • Nicola Elvassore

    (University College London GOS Institute of Child Health
    ShanghaiTech University
    University of Padova)

  • Paolo De Coppi

    (University College London GOS Institute of Child Health
    Great Ormond Street Hospital)

Abstract

Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

Suggested Citation

  • Giovanni Giuseppe Giobbe & Claire Crowley & Camilla Luni & Sara Campinoti & Moustafa Khedr & Kai Kretzschmar & Martina Maria De Santis & Elisa Zambaiti & Federica Michielin & Laween Meran & Qianjiang , 2019. "Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13605-4
    DOI: 10.1038/s41467-019-13605-4
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    Cited by:

    1. Suran Kim & Sungjin Min & Yi Sun Choi & Sung-Hyun Jo & Jae Hun Jung & Kyusun Han & Jin Kim & Soohwan An & Yong Woo Ji & Yun-Gon Kim & Seung-Woo Cho, 2022. "Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids," Nature Communications, Nature, vol. 13(1), pages 1-21, December.
    2. Anna Urciuolo & Giovanni Giuseppe Giobbe & Yixiao Dong & Federica Michielin & Luca Brandolino & Michael Magnussen & Onelia Gagliano & Giulia Selmin & Valentina Scattolini & Paolo Raffa & Paola Caccin , 2023. "Hydrogel-in-hydrogel live bioprinting for guidance and control of organoids and organotypic cultures," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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