IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-08319-6.html
   My bibliography  Save this article

Near-atomic structure of a giant virus

Author

Listed:
  • Qianglin Fang

    (Purdue University)

  • Dongjie Zhu

    (CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
    University of Science and Technology of China)

  • Irina Agarkova

    (University of Nebraska-Lincoln)

  • Jagat Adhikari

    (Washington University)

  • Thomas Klose

    (Purdue University)

  • Yue Liu

    (Purdue University)

  • Zhenguo Chen

    (Purdue University)

  • Yingyuan Sun

    (Purdue University)

  • Michael L. Gross

    (Washington University)

  • James L. Van Etten

    (University of Nebraska-Lincoln)

  • Xinzheng Zhang

    (CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
    Academy of Sciences)

  • Michael G. Rossmann

    (Purdue University)

Abstract

Although the nucleocytoplasmic large DNA viruses (NCLDVs) are one of the largest group of viruses that infect many eukaryotic hosts, the near-atomic resolution structures of these viruses have remained unknown. Here we describe a 3.5 Å resolution icosahedrally averaged capsid structure of Paramecium bursaria chlorella virus 1 (PBCV-1). This structure consists of 5040 copies of the major capsid protein, 60 copies of the penton protein and 1800 minor capsid proteins of which there are 13 different types. The minor capsid proteins form a hexagonal network below the outer capsid shell, stabilizing the capsid by binding neighboring capsomers together. The size of the viral capsid is determined by a tape-measure, minor capsid protein of which there are 60 copies in the virion. Homologs of the tape-measure protein and some of the other minor capsid proteins exist in other NCLDVs. Thus, a similar capsid assembly pathway might be used by other NCLDVs.

Suggested Citation

  • Qianglin Fang & Dongjie Zhu & Irina Agarkova & Jagat Adhikari & Thomas Klose & Yue Liu & Zhenguo Chen & Yingyuan Sun & Michael L. Gross & James L. Van Etten & Xinzheng Zhang & Michael G. Rossmann, 2019. "Near-atomic structure of a giant virus," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08319-6
    DOI: 10.1038/s41467-019-08319-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-08319-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-08319-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Qianqian Shao & Irina V. Agarkova & Eric A. Noel & David D. Dunigan & Yunshu Liu & Aohan Wang & Mingcheng Guo & Linlin Xie & Xinyue Zhao & Michael G. Rossmann & James L. Etten & Thomas Klose & Qiangli, 2022. "Near-atomic, non-icosahedrally averaged structure of giant virus Paramecium bursaria chlorella virus 1," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Zhennan Zhao & Youhua Huang & Congcong Liu & Dongjie Zhu & Shuaixin Gao & Sheng Liu & Ruchao Peng & Ya Zhang & Xiaohong Huang & Jianxun Qi & Catherine C. L. Wong & Xinzheng Zhang & Peiyi Wang & Qiwei , 2023. "Near-atomic architecture of Singapore grouper iridovirus and implications for giant virus assembly," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Jaekyung Hyun & Hideyuki Matsunami & Tae Gyun Kim & Matthias Wolf, 2022. "Assembly mechanism of the pleomorphic immature poxvirus scaffold," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08319-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.