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Near-atomic structure of a giant virus

Author

Listed:
  • Qianglin Fang

    (Purdue University)

  • Dongjie Zhu

    (CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
    University of Science and Technology of China)

  • Irina Agarkova

    (University of Nebraska-Lincoln)

  • Jagat Adhikari

    (Washington University)

  • Thomas Klose

    (Purdue University)

  • Yue Liu

    (Purdue University)

  • Zhenguo Chen

    (Purdue University)

  • Yingyuan Sun

    (Purdue University)

  • Michael L. Gross

    (Washington University)

  • James L. Van Etten

    (University of Nebraska-Lincoln)

  • Xinzheng Zhang

    (CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
    Academy of Sciences)

  • Michael G. Rossmann

    (Purdue University)

Abstract

Although the nucleocytoplasmic large DNA viruses (NCLDVs) are one of the largest group of viruses that infect many eukaryotic hosts, the near-atomic resolution structures of these viruses have remained unknown. Here we describe a 3.5 Å resolution icosahedrally averaged capsid structure of Paramecium bursaria chlorella virus 1 (PBCV-1). This structure consists of 5040 copies of the major capsid protein, 60 copies of the penton protein and 1800 minor capsid proteins of which there are 13 different types. The minor capsid proteins form a hexagonal network below the outer capsid shell, stabilizing the capsid by binding neighboring capsomers together. The size of the viral capsid is determined by a tape-measure, minor capsid protein of which there are 60 copies in the virion. Homologs of the tape-measure protein and some of the other minor capsid proteins exist in other NCLDVs. Thus, a similar capsid assembly pathway might be used by other NCLDVs.

Suggested Citation

  • Qianglin Fang & Dongjie Zhu & Irina Agarkova & Jagat Adhikari & Thomas Klose & Yue Liu & Zhenguo Chen & Yingyuan Sun & Michael L. Gross & James L. Van Etten & Xinzheng Zhang & Michael G. Rossmann, 2019. "Near-atomic structure of a giant virus," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08319-6
    DOI: 10.1038/s41467-019-08319-6
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    Cited by:

    1. Yashan Yang & Qianqian Shao & Mingcheng Guo & Lin Han & Xinyue Zhao & Aohan Wang & Xiangyun Li & Bo Wang & Ji-An Pan & Zhenguo Chen & Andrei Fokine & Lei Sun & Qianglin Fang, 2024. "Capsid structure of bacteriophage ΦKZ provides insights into assembly and stabilization of jumbo phages," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Qianqian Shao & Irina V. Agarkova & Eric A. Noel & David D. Dunigan & Yunshu Liu & Aohan Wang & Mingcheng Guo & Linlin Xie & Xinyue Zhao & Michael G. Rossmann & James L. Etten & Thomas Klose & Qiangli, 2022. "Near-atomic, non-icosahedrally averaged structure of giant virus Paramecium bursaria chlorella virus 1," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    3. Zhennan Zhao & Youhua Huang & Congcong Liu & Dongjie Zhu & Shuaixin Gao & Sheng Liu & Ruchao Peng & Ya Zhang & Xiaohong Huang & Jianxun Qi & Catherine C. L. Wong & Xinzheng Zhang & Peiyi Wang & Qiwei , 2023. "Near-atomic architecture of Singapore grouper iridovirus and implications for giant virus assembly," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    4. Jaekyung Hyun & Hideyuki Matsunami & Tae Gyun Kim & Matthias Wolf, 2022. "Assembly mechanism of the pleomorphic immature poxvirus scaffold," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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