IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-36644-4.html
   My bibliography  Save this article

Asymmetric activity of NetrinB controls laterality of the Drosophila brain

Author

Listed:
  • F. Lapraz

    (Université Côte d’Azur, CNRS, Inserm, iBV)

  • C. Boutres

    (Université Côte d’Azur, CNRS, Inserm, iBV)

  • C. Fixary-Schuster

    (Université Côte d’Azur, CNRS, Inserm, iBV)

  • B. R. Queiroz

    (Université Côte d’Azur, CNRS, Inserm, iBV)

  • P. Y. Plaçais

    (PSL Research University)

  • D. Cerezo

    (Université Côte d’Azur, CNRS, Inserm, iBV)

  • F. Besse

    (Université Côte d’Azur, CNRS, Inserm, iBV)

  • T. Préat

    (PSL Research University)

  • S. Noselli

    (Université Côte d’Azur, CNRS, Inserm, iBV)

Abstract

Left-Right (LR) asymmetry of the nervous system is widespread across animals and is thought to be important for cognition and behaviour. But in contrast to visceral organ asymmetry, the genetic basis and function of brain laterality remain only poorly characterized. In this study, we performed RNAi screening to identify genes controlling brain asymmetry in Drosophila. We found that the conserved NetrinB (NetB) pathway is required for a small group of bilateral neurons to project asymmetrically into a pair of neuropils (Asymmetrical Bodies, AB) in the central brain in both sexes. While neurons project unilaterally into the right AB in wild-type flies, netB mutants show a bilateral projection phenotype and hence lose asymmetry. Developmental time course analysis reveals an initially bilateral connectivity, eventually resolving into a right asymmetrical circuit during metamorphosis, with the NetB pathway being required just prior symmetry breaking. We show using unilateral clonal analysis that netB activity is required specifically on the right side for neurons to innervate the right AB. We finally show that loss of NetB pathway activity leads to specific alteration of long-term memory, providing a functional link between asymmetrical circuitry determined by NetB and animal cognitive functions.

Suggested Citation

  • F. Lapraz & C. Boutres & C. Fixary-Schuster & B. R. Queiroz & P. Y. Plaçais & D. Cerezo & F. Besse & T. Préat & S. Noselli, 2023. "Asymmetric activity of NetrinB controls laterality of the Drosophila brain," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36644-4
    DOI: 10.1038/s41467-023-36644-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-36644-4
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-36644-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Alberto Pascual & Kai-Lian Huang & Julie Neveu & Thomas Préat, 2004. "Brain asymmetry and long-term memory," Nature, Nature, vol. 427(6975), pages 605-606, February.
    2. Pauline Spéder & Géza Ádám & Stéphane Noselli, 2006. "Type ID unconventional myosin controls left–right asymmetry in Drosophila," Nature, Nature, vol. 440(7085), pages 803-807, April.
    3. Chloé Dominici & Juan Antonio Moreno-Bravo & Sergi Roig Puiggros & Quentin Rappeneau & Nicolas Rama & Pauline Vieugue & Agns Bernet & Patrick Mehlen & Alain Chédotal, 2017. "Floor-plate-derived netrin-1 is dispensable for commissural axon guidance," Nature, Nature, vol. 545(7654), pages 350-354, May.
    4. Shunya Hozumi & Reo Maeda & Kiichiro Taniguchi & Maiko Kanai & Syuichi Shirakabe & Takeshi Sasamura & Pauline Spéder & Stéphane Noselli & Toshiro Aigaki & Ryutaro Murakami & Kenji Matsuno, 2006. "An unconventional myosin in Drosophila reverses the default handedness in visceral organs," Nature, Nature, vol. 440(7085), pages 798-802, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Akshai Janardhana Kurup & Florian Bailet & Maximilian Fürthauer, 2024. "Myosin1G promotes Nodal signaling to control zebrafish left-right asymmetry," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    2. Yee Han Tee & Wei Jia Goh & Xianbin Yong & Hui Ting Ong & Jinrong Hu & Ignacius Yan Yun Tay & Shidong Shi & Salma Jalal & Samuel F. H. Barnett & Pakorn Kanchanawong & Wenmao Huang & Jie Yan & Yong Ann, 2023. "Actin polymerisation and crosslinking drive left-right asymmetry in single cell and cell collectives," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Lin, Hairong & Wang, Chunhua & Sun, Jingru & Zhang, Xin & Sun, Yichuang & Iu, Herbert H.C., 2023. "Memristor-coupled asymmetric neural networks: Bionic modeling, chaotic dynamics analysis and encryption application," Chaos, Solitons & Fractals, Elsevier, vol. 166(C).

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36644-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.