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A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells

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  • Kazuki Obashi

    (National Institutes of Health)

  • Kem A. Sochacki

    (National Institutes of Health)

  • Marie-Paule Strub

    (National Institutes of Health)

  • Justin W. Taraska

    (National Institutes of Health)

Abstract

Conformational changes in endocytic proteins are regulators of clathrin-mediated endocytosis. Three clathrin heavy chains associated with clathrin light chains (CLC) assemble into triskelia that link into a geometric lattice that curves to drive endocytosis. Structural changes in CLC have been shown to regulate triskelia assembly in solution, yet the nature of these changes, and their effects on lattice growth, curvature, and endocytosis in cells are unknown. Here, we develop a new correlative fluorescence resonance energy transfer (FRET) and platinum replica electron microscopy method, named FRET-CLEM. With FRET-CLEM, we measure conformational changes in clathrin at thousands of individual morphologically distinct clathrin-coated structures. We discover that the N-terminus of CLC repositions away from the plasma membrane and triskelia vertex as coats curve. Preventing this conformational switch with chemical tools increases lattice sizes and inhibits endocytosis. Thus, a specific conformational switch in the light chain regulates lattice curvature and endocytosis in mammalian cells.

Suggested Citation

  • Kazuki Obashi & Kem A. Sochacki & Marie-Paule Strub & Justin W. Taraska, 2023. "A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36304-7
    DOI: 10.1038/s41467-023-36304-7
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    References listed on IDEAS

    as
    1. Bijeta Prasai & Gideon J. Haber & Marie-Paule Strub & Regina Ahn & John A. Ciemniecki & Kem A. Sochacki & Justin W. Taraska, 2021. "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Brandon L. Scott & Kem A. Sochacki & Shalini T. Low-Nam & Elizabeth M. Bailey & QuocAhn Luu & Amy Hor & Andrea M. Dickey & Steve Smith & Jason G. Kerkvliet & Justin W. Taraska & Adam D. Hoppe, 2018. "Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    3. Stéphane Vassilopoulos & Solène Gibaud & Angélique Jimenez & Ghislaine Caillol & Christophe Leterrier, 2019. "Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    4. Baron Chanda & Osei Kwame Asamoah & Rikard Blunck & Benoît Roux & Francisco Bezanilla, 2005. "Gating charge displacement in voltage-gated ion channels involves limited transmembrane movement," Nature, Nature, vol. 436(7052), pages 852-856, August.
    5. Alexander Fotin & Yifan Cheng & Piotr Sliz & Nikolaus Grigorieff & Stephen C. Harrison & Tomas Kirchhausen & Thomas Walz, 2004. "Molecular model for a complete clathrin lattice from electron cryomicroscopy," Nature, Nature, vol. 432(7017), pages 573-579, December.
    6. Nicholas I. Clarke & Stephen J. Royle, 2018. "FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    7. Delia Bucher & Felix Frey & Kem A. Sochacki & Susann Kummer & Jan-Philip Bergeest & William J. Godinez & Hans-Georg Kräusslich & Karl Rohr & Justin W. Taraska & Ulrich S. Schwarz & Steeve Boulant, 2018. "Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    8. Matteo Biancospino & Gwen R. Buel & Carlos A. Niño & Elena Maspero & Rossella Scotto di Perrotolo & Andrea Raimondi & Lisa Redlingshöfer & Janine Weber & Frances M. Brodsky & Kylie J. Walters & Simona, 2019. "Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
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