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Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings

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  • Stéphane Vassilopoulos

    (Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974)

  • Solène Gibaud

    (Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto)

  • Angélique Jimenez

    (Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto)

  • Ghislaine Caillol

    (Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto)

  • Christophe Leterrier

    (Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto)

Abstract

Recent super-resolution microscopy studies have unveiled a periodic scaffold of actin rings regularly spaced by spectrins under the plasma membrane of axons. However, ultrastructural details are unknown, limiting a molecular and mechanistic understanding of these enigmatic structures. Here, we combine platinum-replica electron and optical super-resolution microscopy to investigate the cortical cytoskeleton of axons at the ultrastructural level. Immunogold labeling and correlative super-resolution/electron microscopy allow us to unambiguously resolve actin rings as braids made of two long, intertwined actin filaments connected by a dense mesh of aligned spectrins. This molecular arrangement contrasts with the currently assumed model of actin rings made of short, capped actin filaments. Along the proximal axon, we resolved the presence of phospho-myosin light chain and the scaffold connection with microtubules via ankyrin G. We propose that braided rings explain the observed stability of the actin-spectrin scaffold and ultimately participate in preserving the axon integrity.

Suggested Citation

  • Stéphane Vassilopoulos & Solène Gibaud & Angélique Jimenez & Ghislaine Caillol & Christophe Leterrier, 2019. "Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13835-6
    DOI: 10.1038/s41467-019-13835-6
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    Cited by:

    1. Andrea Ghisleni & Mayte Bonilla-Quintana & Michele Crestani & Zeno Lavagnino & Camilla Galli & Padmini Rangamani & Nils C. Gauthier, 2024. "Mechanically induced topological transition of spectrin regulates its distribution in the mammalian cell cortex," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    2. Ruobo Zhou & Boran Han & Roberta Nowak & Yunzhe Lu & Evan Heller & Chenglong Xia & Athar H. Chishti & Velia M. Fowler & Xiaowei Zhuang, 2022. "Proteomic and functional analyses of the periodic membrane skeleton in neurons," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Kazuki Obashi & Kem A. Sochacki & Marie-Paule Strub & Justin W. Taraska, 2023. "A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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